中华眼外伤职业眼病杂志
中華眼外傷職業眼病雜誌
중화안외상직업안병잡지
CHINESE JOURNAL OF OCULAR TRAUMA AND OCCUPATIONAL EYE DISEASE
2012年
1期
2-5
,共4页
黄波%顾宝文%应方薇%胡文学%龙巧燕
黃波%顧寶文%應方薇%鬍文學%龍巧燕
황파%고보문%응방미%호문학%룡교연
人参皂苷Rg 1%视神经损伤%神经保护药物
人參皂苷Rg 1%視神經損傷%神經保護藥物
인삼조감Rg 1%시신경손상%신경보호약물
Ginsenoside Rg 1%Optic nerve injury%Neuroprotective drugs
目的 研究人参皂苷Rg 1对大鼠视神经挫伤的保护作用.方法 制作大鼠视神经挫伤模型,设置实验组和对照组,腹腔注射人参皂苷Rg 1,10 mg/kg,连续注射20 d,对照组腹腔注射与实验组等体积生理盐水.20 d后F-VEP检查.取实验组和对照组的视神经挫伤眼和对照眼的眼球及球后视神经:HE染色,SABC法免疫组织化学染色:Bcl-2,Neurocan统计学分析.结果 F-VEP:实验组和对照组的损伤眼数据之间差异较大,但按统计学处理,差异无统计学意义,可能与样本较小及随访时间较短等有关,尚待进一步研究.HE染色健康大鼠视神经组织细胞和视神经挫伤组形态学有差异.免疫组化提示视神经挫伤后神经细胞凋亡明显增多,人参皂苷Rg 1腹腔注射能够明显改善细胞凋亡情况.视神经挫伤后神经纤维蛋白表达明显增多,可能部分与其改善细胞凋亡情况有关,但机制尚不清楚.结论 人参皂苷Rg 1对大鼠视神经挫伤似有保护作用.
目的 研究人參皂苷Rg 1對大鼠視神經挫傷的保護作用.方法 製作大鼠視神經挫傷模型,設置實驗組和對照組,腹腔註射人參皂苷Rg 1,10 mg/kg,連續註射20 d,對照組腹腔註射與實驗組等體積生理鹽水.20 d後F-VEP檢查.取實驗組和對照組的視神經挫傷眼和對照眼的眼毬及毬後視神經:HE染色,SABC法免疫組織化學染色:Bcl-2,Neurocan統計學分析.結果 F-VEP:實驗組和對照組的損傷眼數據之間差異較大,但按統計學處理,差異無統計學意義,可能與樣本較小及隨訪時間較短等有關,尚待進一步研究.HE染色健康大鼠視神經組織細胞和視神經挫傷組形態學有差異.免疫組化提示視神經挫傷後神經細胞凋亡明顯增多,人參皂苷Rg 1腹腔註射能夠明顯改善細胞凋亡情況.視神經挫傷後神經纖維蛋白錶達明顯增多,可能部分與其改善細胞凋亡情況有關,但機製尚不清楚.結論 人參皂苷Rg 1對大鼠視神經挫傷似有保護作用.
목적 연구인삼조감Rg 1대대서시신경좌상적보호작용.방법 제작대서시신경좌상모형,설치실험조화대조조,복강주사인삼조감Rg 1,10 mg/kg,련속주사20 d,대조조복강주사여실험조등체적생리염수.20 d후F-VEP검사.취실험조화대조조적시신경좌상안화대조안적안구급구후시신경:HE염색,SABC법면역조직화학염색:Bcl-2,Neurocan통계학분석.결과 F-VEP:실험조화대조조적손상안수거지간차이교대,단안통계학처리,차이무통계학의의,가능여양본교소급수방시간교단등유관,상대진일보연구.HE염색건강대서시신경조직세포화시신경좌상조형태학유차이.면역조화제시시신경좌상후신경세포조망명현증다,인삼조감Rg 1복강주사능구명현개선세포조망정황.시신경좌상후신경섬유단백표체명현증다,가능부분여기개선세포조망정황유관,단궤제상불청초.결론 인삼조감Rg 1대대서시신경좌상사유보호작용.
Objective To study the protective effect of ginsenoside Rg 1 on rat optic nerve injury.Methods The models of rat optic nerveinjury were separated into experimental group and control group.performing intraperitoneal injection of ginsenosides Rg 1(10mg/kg)was given to experimental group of rats for continuous 20 days,and the intraperitoneal injection with same volume of saline was given to the control group.F-VEP examination was done.The eyeball and optic nerve were taken out from the optic nerve injury eye and the normal eye in both groups:HE staining,SABC immunohistochemical staining:Bcl-2,Neurocan statistical analysis were done.Results The F-VEP examination result showed the significant difference in the data of injury eye from experimental group and control group,but no significant statistical difference.It needs further study.HE staining result showed the morphological difference on optic nerve cells from health rat group and the optic nerve injury group.Immunohistochemistry showed the neuronal cell apoptosis was significantly increased after optic nerve injury.The intraperitoneal injection of ginsenoside Rg 1 could significantly improve the situation of cell apoptosis.The significant increment of fiber protein may be partly related to the improvement of cell apoptosis,but the principle is not clear.Conclusion Ginsenoside Rg 1 has protective effect on rat optic nerve injury.
