国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2010年
3期
226-229
,共4页
丙泊酚%脂多糖%人单核细胞%Peroxiredoxin-2%超氧化物歧化酶
丙泊酚%脂多糖%人單覈細胞%Peroxiredoxin-2%超氧化物歧化酶
병박분%지다당%인단핵세포%Peroxiredoxin-2%초양화물기화매
Propofoll%Lipopolysaccharide%Human mononuclear macrophage cell%Peroxiredoxin-2%SOD1
目的 研究丙泊酚对脂多糖(lipopolysaccharide,LPS)诱导人单核细胞(THP-1)表达Peroxiredoxin-2及超氧化物歧化酶(SOD1)的影响.方法 将体外培养的THP-1细胞按照完全随机方法分为4组:正常对照组(C组);LPS刺激组(L组):加入LPS 1 mg/L;丙泊酚处理组(P组):给予丙泊酚15 mg/L和丙泊酚预处理合并LPS刺激组(P+L组):给予丙泊酚15 mg/L,1 h后再给予LPS 1 mg/L.通过Western blot法分别检测各组内Peroxiredoxin-2及SOD1含量的变化,用四唑盐快速比色法(MTT)观察各组THP-1细胞的存活率.结果 在LPS刺激12 h后收集细胞,Westernblot结果显示与正常对照组(C组)、丙泊酚处理组(P组)、丙泊酚预处理合并LP3刺激组(P+L组)3组相比,LPS刺激组(L组)中Peroxiredoxin-2和SOD1的表达量明显降低,而L组与(P+L)组比较,(P+L)组中Peroxiredoxin-2和SOD1的表达量则明显高于L组;MTT结果显示L组与(L+P)组相比较,L组细胞存活率明显低于(L+P)组细胞存活率(P<0.05)结论丙泊酚可以逆转LPS对THP1细胞表达抗氧化蛋白Peroxiredoxin-2及SOD1的抑制作用,其抗氧化作用可能与增加Peroxiredoxin-2及SOD1的表达有关.
目的 研究丙泊酚對脂多糖(lipopolysaccharide,LPS)誘導人單覈細胞(THP-1)錶達Peroxiredoxin-2及超氧化物歧化酶(SOD1)的影響.方法 將體外培養的THP-1細胞按照完全隨機方法分為4組:正常對照組(C組);LPS刺激組(L組):加入LPS 1 mg/L;丙泊酚處理組(P組):給予丙泊酚15 mg/L和丙泊酚預處理閤併LPS刺激組(P+L組):給予丙泊酚15 mg/L,1 h後再給予LPS 1 mg/L.通過Western blot法分彆檢測各組內Peroxiredoxin-2及SOD1含量的變化,用四唑鹽快速比色法(MTT)觀察各組THP-1細胞的存活率.結果 在LPS刺激12 h後收集細胞,Westernblot結果顯示與正常對照組(C組)、丙泊酚處理組(P組)、丙泊酚預處理閤併LP3刺激組(P+L組)3組相比,LPS刺激組(L組)中Peroxiredoxin-2和SOD1的錶達量明顯降低,而L組與(P+L)組比較,(P+L)組中Peroxiredoxin-2和SOD1的錶達量則明顯高于L組;MTT結果顯示L組與(L+P)組相比較,L組細胞存活率明顯低于(L+P)組細胞存活率(P<0.05)結論丙泊酚可以逆轉LPS對THP1細胞錶達抗氧化蛋白Peroxiredoxin-2及SOD1的抑製作用,其抗氧化作用可能與增加Peroxiredoxin-2及SOD1的錶達有關.
목적 연구병박분대지다당(lipopolysaccharide,LPS)유도인단핵세포(THP-1)표체Peroxiredoxin-2급초양화물기화매(SOD1)적영향.방법 장체외배양적THP-1세포안조완전수궤방법분위4조:정상대조조(C조);LPS자격조(L조):가입LPS 1 mg/L;병박분처리조(P조):급여병박분15 mg/L화병박분예처리합병LPS자격조(P+L조):급여병박분15 mg/L,1 h후재급여LPS 1 mg/L.통과Western blot법분별검측각조내Peroxiredoxin-2급SOD1함량적변화,용사서염쾌속비색법(MTT)관찰각조THP-1세포적존활솔.결과 재LPS자격12 h후수집세포,Westernblot결과현시여정상대조조(C조)、병박분처리조(P조)、병박분예처리합병LP3자격조(P+L조)3조상비,LPS자격조(L조)중Peroxiredoxin-2화SOD1적표체량명현강저,이L조여(P+L)조비교,(P+L)조중Peroxiredoxin-2화SOD1적표체량칙명현고우L조;MTT결과현시L조여(L+P)조상비교,L조세포존활솔명현저우(L+P)조세포존활솔(P<0.05)결론병박분가이역전LPS대THP1세포표체항양화단백Peroxiredoxin-2급SOD1적억제작용,기항양화작용가능여증가Peroxiredoxin-2급SOD1적표체유관.
Objective To explore effect of propofol on Peroxiredoxin -2 and SOD1 expression from human mononuclear macrophage cell (THP-1 )induced by lipopolysaccharide (LPS). Methods THP-1 cultured in vitro and they were randomly assigned to four groups: control group, LPS group(L group): LPS 1 mg/L; propofol group(P group): propofol 15 mg/L, LPS and pretreatment with propofol group (P+L group): propofol 15 mg/L,after 1 hour, adding LPS 1 mg/L. Using western blot to detect the expression of Peroxiredoxin-2 and SOD1, and using MTT to observe the survival rate of cell. Results 12 h after stimulated by LPS, results in Westernblot show that the expression of Peroxiredoxin-2 and SOD1 were both significantly decreased in L group compared to other three groups, however Peroxiredoxin-2 and SOD1 were both higher in L+P group than in L group. Results in MTT show that the survival rate of cell in L group was significantly lower than LPS and pretreatment with propofol group (P<0.05). Conclusion Propofol can increase the expression of Peroxiredoxin-2 and SOD1, and it may have some anti-peroxidation effects.
