国际流行病学传染病学杂志
國際流行病學傳染病學雜誌
국제류행병학전염병학잡지
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY AND INFECTIOUS DISEASE
2012年
2期
92-94
,共3页
陈群伟%潘宏仪%孙洪运%徐晶%谌翠容%尚世强%叶荣夏%娄国强%潘红英
陳群偉%潘宏儀%孫洪運%徐晶%諶翠容%尚世彊%葉榮夏%婁國彊%潘紅英
진군위%반굉의%손홍운%서정%심취용%상세강%협영하%루국강%반홍영
寡核苷酸序列分析%腹水%16S rRNA基因%自发性细菌性腹膜炎%抗生素
寡覈苷痠序列分析%腹水%16S rRNA基因%自髮性細菌性腹膜炎%抗生素
과핵감산서렬분석%복수%16S rRNA기인%자발성세균성복막염%항생소
Oligonucleotide array sequence analysis%Ascites%16S rRNA genes%Spontaneous bacterial peritonitis%Antibiotics
目的 评价抗生素的应用是否影响基因芯片(寡核苷酸序列分析)检测腹水细菌16S rRNA 基因在自发性细菌性腹膜炎(SBP)诊断中的应用.方法 采用16S rRNA PCR-基因芯片检测76例临床疑似SBP肝病患者的腹水细菌16S rRNA基因,与同期患者的腹水细菌培养相比,分析两种不同检测方法对应用抗生素(31例)和未应用抗生素(45例)患者的细菌阳性率的差异.结果 76份疑似SBP患者的腹水样本中,基因芯片检测阳性率22.37％(17份),明显高于腹水细菌培养阳性率的7.89％(6份),两种方法差异有统计学意义( x2=18.05,P＜0.01).应用抗生素组腹水细菌基因芯片检测阳性率为19.35％(6份),略低于未应用抗生素组的24.44％(11份),但两组差异无统计学意义( x2=0.274,P＞ 0.05).应用抗生素组腹水细菌培养阳性率为0(0/31),而未应用抗生素组阳性率为13.33％(6/45),两组差异有统计学意义(x2=4.488,P＜0.05).结论 16S rRNA PCR-基因芯片检测腹水细菌与腹水培养相比可能更少受抗生素应用的影响.
目的 評價抗生素的應用是否影響基因芯片(寡覈苷痠序列分析)檢測腹水細菌16S rRNA 基因在自髮性細菌性腹膜炎(SBP)診斷中的應用.方法 採用16S rRNA PCR-基因芯片檢測76例臨床疑似SBP肝病患者的腹水細菌16S rRNA基因,與同期患者的腹水細菌培養相比,分析兩種不同檢測方法對應用抗生素(31例)和未應用抗生素(45例)患者的細菌暘性率的差異.結果 76份疑似SBP患者的腹水樣本中,基因芯片檢測暘性率22.37％(17份),明顯高于腹水細菌培養暘性率的7.89％(6份),兩種方法差異有統計學意義( x2=18.05,P＜0.01).應用抗生素組腹水細菌基因芯片檢測暘性率為19.35％(6份),略低于未應用抗生素組的24.44％(11份),但兩組差異無統計學意義( x2=0.274,P＞ 0.05).應用抗生素組腹水細菌培養暘性率為0(0/31),而未應用抗生素組暘性率為13.33％(6/45),兩組差異有統計學意義(x2=4.488,P＜0.05).結論 16S rRNA PCR-基因芯片檢測腹水細菌與腹水培養相比可能更少受抗生素應用的影響.
목적 평개항생소적응용시부영향기인심편(과핵감산서렬분석)검측복수세균16S rRNA 기인재자발성세균성복막염(SBP)진단중적응용.방법 채용16S rRNA PCR-기인심편검측76례림상의사SBP간병환자적복수세균16S rRNA기인,여동기환자적복수세균배양상비,분석량충불동검측방법대응용항생소(31례)화미응용항생소(45례)환자적세균양성솔적차이.결과 76빈의사SBP환자적복수양본중,기인심편검측양성솔22.37％(17빈),명현고우복수세균배양양성솔적7.89％(6빈),량충방법차이유통계학의의( x2=18.05,P＜0.01).응용항생소조복수세균기인심편검측양성솔위19.35％(6빈),략저우미응용항생소조적24.44％(11빈),단량조차이무통계학의의( x2=0.274,P＞ 0.05).응용항생소조복수세균배양양성솔위0(0/31),이미응용항생소조양성솔위13.33％(6/45),량조차이유통계학의의(x2=4.488,P＜0.05).결론 16S rRNA PCR-기인심편검측복수세균여복수배양상비가능경소수항생소응용적영향.
Objective To evaluate the influence to detect ascites bacteria with 16S rRNA PCR-microarray in patients with spontaneous bacterial peritonitis( SBP) after treated with antibiotics.Methods Compared to ascites bacterial culture,ascites bacterial 16S rRNA genes were detected by PCR-microarray in 76 cases of suspected SBP with chronic liver diseases,and the difference of ascites bacteria positive rates between two groups with and without antibiotics were analyzed.Results Of the 76 ascites samples in SBP patients,there were 17(22.37％ ) ascites bacteria positive detected by PCR-microarray,and 6(7.89％ ) ascites bacteria positive detected by bacterial culture.The positive rates of former were much higher(x2 =18.05,P ＜0.01).In groups with and without antibiotics,the positive rates of bacterial detection with PCR-microarray were 19.35％ and 24.44％ respectirely,there was no statistical difference between the two groups( x2 =0.274,P ＞ 0.05) ; and the positive rates of bacterial detection with bacterial culture were 0(0/31 ) and 13.33％(6/45) respectively,there was statistical difference between the two groups(x2 =4.488,P ＜ 0.05).Conclusions Compared to the ascites bacterial culture,less influence is found in ascites bacteria detection with 16S rRNA PCR-microarray in patients with SBP after treatment with antibiotics.
