中国药学（英文版）
中國藥學（英文版）
중국약학（영문판）
JOURNAL OF CHINESE PHARMACEUTICAL SCIENCES
2007年
2期
146-152
,共7页
王陆军%岳鹏飞%赵艳玲%蔡培烈%周旭%袁海龙
王陸軍%嶽鵬飛%趙豔玲%蔡培烈%週旭%袁海龍
왕륙군%악붕비%조염령%채배렬%주욱%원해룡
苦参素磷脂复合物%2.2.15 cell line%HBV-DNA
苦參素燐脂複閤物%2.2.15 cell line%HBV-DNA
고삼소린지복합물%2.2.15 cell line%HBV-DNA
Oxymatrine phospholipid complex%2.2.15 Cell line%HBV-DNA
目的 制备苦参素磷脂复合物并对其体外抗乙肝病毒活性进行评价.方法 利用四氢呋喃作为反应溶剂,苦参素及磷脂溶于溶媒中,真空除去有机溶剂得苦参素磷脂复合物.并以2.2.15 cell line为细胞模型,分别对苦参素磷脂复合物的细胞毒性值和乙型肝炎表面抗原,乙型肝炎e抗原,乙型肝炎病毒DNA的抑制率进行了研究.结果 制得的苦参素磷脂复合物中苦参素的含量为24.86%.苦参素磷脂复合物的细胞毒性值为250 μg·mL-1.与苦参素相比,苦参素磷脂复合物对2.2.15细胞的HBsAg,HBeAg,HBV-DNA值明显降低.结论 苦参素磷脂复合物能够产生更有效的抗乙肝病毒活性.因此乙肝治疗中苦参素磷脂复合物有很强的抗病毒活性潜力.
目的 製備苦參素燐脂複閤物併對其體外抗乙肝病毒活性進行評價.方法 利用四氫呋喃作為反應溶劑,苦參素及燐脂溶于溶媒中,真空除去有機溶劑得苦參素燐脂複閤物.併以2.2.15 cell line為細胞模型,分彆對苦參素燐脂複閤物的細胞毒性值和乙型肝炎錶麵抗原,乙型肝炎e抗原,乙型肝炎病毒DNA的抑製率進行瞭研究.結果 製得的苦參素燐脂複閤物中苦參素的含量為24.86%.苦參素燐脂複閤物的細胞毒性值為250 μg·mL-1.與苦參素相比,苦參素燐脂複閤物對2.2.15細胞的HBsAg,HBeAg,HBV-DNA值明顯降低.結論 苦參素燐脂複閤物能夠產生更有效的抗乙肝病毒活性.因此乙肝治療中苦參素燐脂複閤物有很彊的抗病毒活性潛力.
목적 제비고삼소린지복합물병대기체외항을간병독활성진행평개.방법 이용사경부남작위반응용제,고삼소급린지용우용매중,진공제거유궤용제득고삼소린지복합물.병이2.2.15 cell line위세포모형,분별대고삼소린지복합물적세포독성치화을형간염표면항원,을형간염e항원,을형간염병독DNA적억제솔진행료연구.결과 제득적고삼소린지복합물중고삼소적함량위24.86%.고삼소린지복합물적세포독성치위250 μg·mL-1.여고삼소상비,고삼소린지복합물대2.2.15세포적HBsAg,HBeAg,HBV-DNA치명현강저.결론 고삼소린지복합물능구산생경유효적항을간병독활성.인차을간치료중고삼소린지복합물유흔강적항병독활성잠력.
Aim The oxymatrine phospholipid complexs were prepared, and its activity against hepatitis B virus in vitro were studied. Methods Using tetrahydrofuran as a reaction medium, oxymatrine and phospholipids were resolved into the medium. Oxymatrine phospholipid complexs were prepared. The toxicity measurements and inhibitory effect on HBsAg, HBeAg, and HBV-DNA of oxymatrine phospholipid complex in 2.2.15 cells were studied respectively. Results The content of oxymatrine in the phospholipids complexs prepared was 24.86% (WAV). The TCO of the oxymatrine phospholipid complexs was 250 μmol·L-1. The inhibitory effect of HBsAg, HBeAg, HBV-DNA of 2.2.15 cells treated by oxymatrine phospholipid complex were higher than those of the oxymatrine. Conclusion Oxymatrine phospholipid complex can have stronger effective activity against hepatitis B virus compared with oxymatrine. So oxymatrine phospholipid complexs were showing its potential antiviral activity in hepatitis B treatment.
