CAJ | 학술논문

研究不同类型培养基、诱导时间、菌体密度和不同补料对rhIFN-γ表达的影响,并运用高密度发酵工艺对rhIFN-γ的产量进行优化,目的蛋白表达量从4 000 mg/L提高到20 000 mg/L以上,发酵产量提高了5倍,大大提高了效率。同时为验证工艺的稳定性,将优化的发酵条件进行连续三批重复发酵实验。结果表明该优化条件具有重复性,表达稳定,维持在40%左右,从而为rhIFN-γ大规模生产奠定了良好的基础。
연구불동류형배양기、유도시간、균체밀도화불동보료대rhIFN-γ표체적영향,병운용고밀도발효공예대rhIFN-γ적산량진행우화,목적단백표체량종4 000 mg/L제고도20 000 mg/L이상,발효산량제고료5배,대대제고료효솔。동시위험증공예적은정성,장우화적발효조건진행련속삼비중복발효실험。결과표명해우화조건구유중복성,표체은정,유지재40%좌우,종이위rhIFN-γ대규모생산전정료량호적기출。
The expression level of rhIFN-γ was influenced by the different medium,induction time,cell density and feeding batch.The production of rhIFN-γ was optimized by high density fermentation.The expression level of rhIFN-γ was increased from 4 000 mg/L to over 20 000 mg/L,the fermentation yield was increased by 5 times.In order to confirm the technical stability,a three-time repeated fermentation was performed.The result indicated that the optimal conditions had the repeatability and the expression level remained 40% or so.This fermentation technology established a good foundation for the rhIFN-γ mass production.