河北化工
河北化工
하북화공
HEBEI CHEMICAL ENGINEERING AND INDUSTRY
2011年
11期
33-34,46
,共3页
化学合成%白藜芦醇%高效液相色谱法
化學閤成%白藜蘆醇%高效液相色譜法
화학합성%백려호순%고효액상색보법
synthesis%resveratrol%HPLC
建立了一种检测转化学合成白藜芦醇的高效液相色谱法。采用250×4.6 mm、5μm的C18色谱柱,流动相为乙腈-0.1%乙酸（40∶60）,流速为1.0 mL/min,进样量为10μL,波长为306 nm,检测器为PDA。实验结果表明,该方法线性关系良好,白藜芦醇相关系数r=0.999 9,回收率为98.0%～100.8%,准确度高。解决了化学合成白藜芦醇的检测难题,具有实际应用价值。
建立瞭一種檢測轉化學閤成白藜蘆醇的高效液相色譜法。採用250×4.6 mm、5μm的C18色譜柱,流動相為乙腈-0.1%乙痠（40∶60）,流速為1.0 mL/min,進樣量為10μL,波長為306 nm,檢測器為PDA。實驗結果錶明,該方法線性關繫良好,白藜蘆醇相關繫數r=0.999 9,迴收率為98.0%～100.8%,準確度高。解決瞭化學閤成白藜蘆醇的檢測難題,具有實際應用價值。
건립료일충검측전화학합성백려호순적고효액상색보법。채용250×4.6 mm、5μm적C18색보주,류동상위을정-0.1%을산（40∶60）,류속위1.0 mL/min,진양량위10μL,파장위306 nm,검측기위PDA。실험결과표명,해방법선성관계량호,백려호순상관계수r=0.999 9,회수솔위98.0%～100.8%,준학도고。해결료화학합성백려호순적검측난제,구유실제응용개치。
A method was established to determine the content of synthetic resveratrol by high performance liquid chromatography（HPLC）.A phenomenex luna-C18 column（250×4.6 mm,5μm） was used with 1.0 mL/min acetonitrile-0.1% acetic acid as mobile phase and detection wave length was 306 nm.Good linear relationship of the calibration curves of resveratrol（r = 0.999 9） was obtained and the recovery rate of resveratrol was 98.0%～100.8%.The method solves the problem of determining synthetic resveratrol,which is of great practical value.
