中国动物传染病学报
中國動物傳染病學報
중국동물전염병학보
CHINESE JOURNAL OF VETERINARY PARASITOLOGY
2011年
5期
45-50
,共6页
胡青海%宋翠萍%于圣青%丁铲%韩先干%屠晶%苗双%卢凤英%吴冬明%糜益锋
鬍青海%宋翠萍%于聖青%丁鏟%韓先榦%屠晶%苗雙%盧鳳英%吳鼕明%糜益鋒
호청해%송취평%우골청%정산%한선간%도정%묘쌍%로봉영%오동명%미익봉
鸭腹水综合征%鸭肝炎病毒%樱桃谷鸭%病毒分离
鴨腹水綜閤徵%鴨肝炎病毒%櫻桃穀鴨%病毒分離
압복수종합정%압간염병독%앵도곡압%병독분리
Duck ascites syndrome%Duck hepatitis virus%cherry valley ducks%virus isolation
2011年4月份,河北省某地一些鸭场饲养的20～45日龄的樱桃谷肉鸭发生了以腹腔中充满大量清亮、茶色或啤酒样腹水为主要病变特征的疾病,即鸭腹水综合征。采集两个不同日龄的病死鸭肝脏样品,接种SPF鸭胚进行病毒分离,获得两个病毒分离物（HB01和HB02）。这两个病毒分离物对鸡、鸭和鹅的红细胞均无血凝活性。RT-PCR或PCR检测表明,HB01和HB02中鸭肝炎病毒检测为阳性,而鸭呼肠孤病毒、鸭圆环病毒、鸭黄病毒等检测均为阴性。将HB02接种5日龄SPF鸭,致死率为40%;对20日龄的鸭不致死。HB02分离物中鸭肝炎病毒的序列分析表明其与鸭肝炎病毒NA株及弱毒疫苗株C80和F64遗传距离较近。根据试验结果,推测鸭肝炎病毒可能是诱发本次鸭腹水综合症的因素之一。
2011年4月份,河北省某地一些鴨場飼養的20～45日齡的櫻桃穀肉鴨髮生瞭以腹腔中充滿大量清亮、茶色或啤酒樣腹水為主要病變特徵的疾病,即鴨腹水綜閤徵。採集兩箇不同日齡的病死鴨肝髒樣品,接種SPF鴨胚進行病毒分離,穫得兩箇病毒分離物（HB01和HB02）。這兩箇病毒分離物對鷄、鴨和鵝的紅細胞均無血凝活性。RT-PCR或PCR檢測錶明,HB01和HB02中鴨肝炎病毒檢測為暘性,而鴨呼腸孤病毒、鴨圓環病毒、鴨黃病毒等檢測均為陰性。將HB02接種5日齡SPF鴨,緻死率為40%;對20日齡的鴨不緻死。HB02分離物中鴨肝炎病毒的序列分析錶明其與鴨肝炎病毒NA株及弱毒疫苗株C80和F64遺傳距離較近。根據試驗結果,推測鴨肝炎病毒可能是誘髮本次鴨腹水綜閤癥的因素之一。
2011년4월빈,하북성모지일사압장사양적20～45일령적앵도곡육압발생료이복강중충만대량청량、다색혹비주양복수위주요병변특정적질병,즉압복수종합정。채집량개불동일령적병사압간장양품,접충SPF압배진행병독분리,획득량개병독분리물（HB01화HB02）。저량개병독분리물대계、압화아적홍세포균무혈응활성。RT-PCR혹PCR검측표명,HB01화HB02중압간염병독검측위양성,이압호장고병독、압원배병독、압황병독등검측균위음성。장HB02접충5일령SPF압,치사솔위40%;대20일령적압불치사。HB02분리물중압간염병독적서렬분석표명기여압간염병독NA주급약독역묘주C80화F64유전거리교근。근거시험결과,추측압간염병독가능시유발본차압복수종합증적인소지일。
In April 2011,a duck ascites syndrome was found in 20-45 day-old Cherry Valley ducks in some duck farm of Hebei province.This disease was characterized by the abdominal cavity filled with a clear,dark brown or beer-like ascites.Two virus isolates named HB01 and HB02 were isolated from duck liver samples which were from two different day-age ducks.This two virus isolates had no hemagglutinating activity with chicken,duck and goose red blood cells.RT-PCR or PCR results showed that Duck hepatitis virus type 1 could be detected in HB01 and HB02,but other eight kinds of duck virus not.Four out of ten ducks died within one week postinoculation when 5 day-old SPF ducks were challenged with HB02,while no ducks died in 20 day-old SPF ducks group.Phylogenetic and evolutionary analysis revealed that HB02 had a close relationship with NA,C80 and FC64 strains based on the alignment of the full genome sequence of different strains.According to the above results,we deduced that duck hepatitis virus（including some vaccine virus） could be one of the important factors to induce duck ascites syndrome occurred in some duck farms of Hebei Prorince this year.
