CAJ | 학술논문

采用聚丙烯酰胺凝胶电泳方法研究了鲤、鲢、鳙、草鱼亲鱼消化道消化酶的种类；用比色法及滴定法分别测其消化酶相对活性。结果表明：鲤、鲢、鳙、草鱼4种鱼消化道的蛋白酶、淀粉酶活性与食性有明显的相关性。其中，蛋白酶活性依次为鲤〉鲢〉鳙〉草鱼；淀粉酶活性依次为草鱼〉鲤〉鳙〉鲢。脂肪酶活性与食性的关系不明显。四种鱼消化酶的表达与消化道的组织结构特征相一致，消化道前2／5部位（G1、G2）的消化酶活性最高，是无胃鱼消化道的主要消化场所。消化道中央部位（G3）的消化酶活性次之，能将未消化完全的食糜进一步消化，而消化道后段（G4、G5）消化酶活性最低。可见，消化酶活性及消化功能呈递减形式，食物在消化道中随着消化、吸收，逐渐排出体外。本研究为鲤、鲢、草鱼、鳙4种鱼的营养生理学、高效饲料配比的研制及种质资源的科学管理提供理论依据。
채용취병희선알응효전영방법연구료리、련、용、초어친어소화도소화매적충류；용비색법급적정법분별측기소화매상대활성。결과표명：리、련、용、초어4충어소화도적단백매、정분매활성여식성유명현적상관성。기중，단백매활성의차위리〉련〉용〉초어；정분매활성의차위초어〉리〉용〉련。지방매활성여식성적관계불명현。사충어소화매적표체여소화도적조직결구특정상일치，소화도전2／5부위（G1、G2）적소화매활성최고，시무위어소화도적주요소화장소。소화도중앙부위（G3）적소화매활성차지，능장미소화완전적식미진일보소화，이소화도후단（G4、G5）소화매활성최저。가견，소화매활성급소화공능정체감형식，식물재소화도중수착소화、흡수，축점배출체외。본연구위리、련、초어、용4충어적영양생이학、고효사료배비적연제급충질자원적과학관리제공이론의거。
The types of digestive enzymes were analyzed in digestive tracts of common carp Cyprinus carpio silver carp Hypophthalmichthys rnolitrix, bigheand carp A ristichthys mobilis, grass carp Ctenopharyngodon idellus by polyacrylamide gel electrophoresis, and the relative activities of the digestive enzymes were determined in the fishes by colorimetry and titration.The results showed that the order of the protease activity was arranged as common carp 〉silver carp 〉 bighead carp 〉 grass carp, and that for amylase activity as grass carp 〉 bighead carp 〉common carp 〉silver carp. The digestive enzyme expression in these fishes was found to be paralleled to the histology structure of digestive tracts. The maximal digestive enzymic activity was observed in the 2/5 parts in anterior end of the digestive tract （G1, G2）, where was found to be the main place for digestion in those stomachless fishes, followed by the following 1/5 part of digestive tract （G3）. The minimal digestive enzymic activity was occurred in the last 2/5 parts （G4, G5） of the intestines. The findings provide a theoretical basis for the nutritional physiology, the development of feedstuff and the management for germplasm in these fisheries.