CAJ | 학술논문

DREB／CBF类转录因子的功能及作用机理在拟南芥、水稻等模式植物中已经得到了较为充分的研究，但是在花生中还没有关于这类基因的报道，其功能也没有得到证实。本研究通过Bioedit软件在花生eDNA文库中找到了15个可能编码CBF类蛋白的基因片段，其中有2个基因没有包含完整的开放阅读框。我们通过5-RACERT-PCR对这两个基因的全长进行了克隆，得到了包含完整读码框的基因序列，并将序列在GenBank上注册，同时对得到的序列进行了初步分析。
DREB／CBF류전록인자적공능급작용궤리재의남개、수도등모식식물중이경득도료교위충분적연구，단시재화생중환몰유관우저류기인적보도，기공능야몰유득도증실。본연구통과Bioedit연건재화생eDNA문고중조도료15개가능편마CBF류단백적기인편단，기중유2개기인몰유포함완정적개방열독광。아문통과5-RACERT-PCR대저량개기인적전장진행료극륭，득도료포함완정독마광적기인서렬，병장서렬재GenBank상주책，동시대득도적서렬진행료초보분석。
The function of DREB/CBF transcript factors had been extensively studied in Arabidopsis and rice. However, no researches had been done in peanut about this kind of gene. In this study, we found 15 ESTs which may encode DREB/CBF proteins from the cDNA library of peanut using the Bioedit software. 5＇ fragments of 2 ESTs were unknown and we amplified the 5＇-end of these two genes through 5＇--RACE RT--PCR. The sequences of the two genes were also submitted to Gen- Bank and analyzed after the whole sequences were cloned.