CAJ | 학술논문

采用不同的供试材料与球孢白僵菌孢子粉混合培养的方法,研究了10种不同的高分子材料与球孢白僵菌分生孢子的生物相容性。结果表明,球孢白僵菌孢子悬浮液与甲基纤维素钠、预胶化淀粉、阿拉伯胶和明胶预混6h后,孢子的发芽率与对照之间无显著性差异;与预胶化淀粉、海藻胶、甲基纤维素钠、明胶、羧甲基纤维素钠和阿拉伯胶在室温下混合贮存12个月后,萌发率仍达75%以上,均显著高于对照;加入明胶、预胶化淀粉和甲基纤维素钠对于菌落的直径生长无显著影响;加有预胶化淀粉、阿拉伯胶、明胶等的培养基产孢量与对照相比无显著性差异。综合比较,相容性能最好的材料依次为预胶化淀粉、明胶和甲基纤维素钠。
채용불동적공시재료여구포백강균포자분혼합배양적방법,연구료10충불동적고분자재료여구포백강균분생포자적생물상용성。결과표명,구포백강균포자현부액여갑기섬유소납、예효화정분、아랍백효화명효예혼6h후,포자적발아솔여대조지간무현저성차이;여예효화정분、해조효、갑기섬유소납、명효、최갑기섬유소납화아랍백효재실온하혼합저존12개월후,맹발솔잉체75%이상,균현저고우대조;가입명효、예효화정분화갑기섬유소납대우균락적직경생장무현저영향;가유예효화정분、아랍백효、명효등적배양기산포량여대조상비무현저성차이。종합비교,상용성능최호적재료의차위예효화정분、명효화갑기섬유소납。
In the present paper,the compatibility of 10 macromolecular materials to conidial viability of Beauveria bassiana was tested by mixing and co-incubation.The results showed that the conidia mixed with methyl cellulose sodium,pre-screened plastic starch,arabic gum and gelatin for 6 h germinated with no significant difference to those of untreated control.The conidia premixed with prescreened plastic starch,alginate jelly,methyl cellulose sodium,gelatin,carboxymethyl cellulose sodium and arabic gum and stored for 12 months at room temperature could germinate up to 75%,significantly higher than those of the control,suggesting a good protection to the conidia.The mixing with gelatin,pre-screened plastic starch and methyl cellulose sodium did not significantly affect fungal growth measured in colony diameter and sporulation.As compared integratedly,the materials with the highest compatibility to the conidia of B.bassiana were in an order of pre-screened plastic starch,gelatin and methyl cellulose sodium.