CAJ | 학술논문

目的:通过改变四氧嘧啶给药次数探索改良制作兔糖尿病模型的方法.方法:选取健康大耳白兔36只,体质量为2～2.5kg ,注射四氧嘧啶剂量均为150mg/kg,随机分为3组,每组12只,分别为一次给药组(A组),分两次给药组(B组)和分三次给药组(C组).观测各组空腹血糖值,计算各组成模率和死亡率.结果:B组兔糖尿病模型成模率显著高于A组(P<0.01),死亡率显著低于A组(P<0.01); C组兔糖尿病模型成模率显著高于B组(P<0.01) ,死亡率显著低于B组(P<0.01).结论:四氧嘧啶分次给药能提高制作兔糖尿病模型的成模率,降低死亡率并且可以保持模型的稳定.
목적:통과개변사양밀정급약차수탐색개량제작토당뇨병모형적방법.방법:선취건강대이백토36지,체질량위2～2.5kg ,주사사양밀정제량균위150mg/kg,수궤분위3조,매조12지,분별위일차급약조(A조),분량차급약조(B조)화분삼차급약조(C조).관측각조공복혈당치,계산각조성모솔화사망솔.결과:B조토당뇨병모형성모솔현저고우A조(P<0.01),사망솔현저저우A조(P<0.01); C조토당뇨병모형성모솔현저고우B조(P<0.01) ,사망솔현저저우B조(P<0.01).결론:사양밀정분차급약능제고제작토당뇨병모형적성모솔,강저사망솔병차가이보지모형적은정.
AIM: To explore the frequency of drug injection of alloxan diabetes on the established model of rabbit.METHODS: Thirty-six healthy rabbits, weighing 2-2.5kg, were randomly divided into one time drug injection group (group A, n=12), two times drug injection group (group B, n=12) and three times drug injection group (group C, n=12). Each rabbit was injected with a total amount of 150mg/kg of alloxan. Fasting blood glucose was measured. The success rate and death rate of each group were also calculated.RESULTS: The success rate of diabetic rabbit model in group B was higher than that in group A (P<0.01) and its death rate was lower than that of group A (P<0.01); the success rate of diabetic rabbit model in group C was highest and the death rate was the lowest in three groups(P<0.01). CONCLUSION: Multiple administration of alloxan can improve success rate in establishing diabetic rabbit model with decreased death rate and increased stability.