CAJ | 학술논문

为研究酚氧化酶(PO)在昆虫蜕皮过程中的功能和作用,采用微量测定法研究了黄粉虫Tenebrio molitor体壁硬化过程中血淋巴和表皮中的PO活性变化.结果表明:初蜕皮幼虫血淋巴中PO活性较高,但随着体壁的不断黑化与硬化,其活性呈现下降趋势,在3～4 h内达到最低点,而后PO活性逐渐上升,7 h左右活性上升至最高,并接近于正常幼虫的水平;在刚蜕完皮后的1 h内,体壁中 PO活性基本无变化,但随后即开始下降,3 h左右降到最低点,然后开始回升,6～7 h左右恢复到正常水平,并趋于稳定;以L-DOPA为底物,通过双倒数曲线作图法求得黄粉虫血淋巴PO的K_m＝1.176 mmol/L,体壁PO的K_m＝0.881 mmol/L,表明体壁PO与底物L-DOPA的亲和力要高于血淋巴PO.研究表明两种来源的酚氧化酶均参与了黄粉虫幼虫的体壁硬化过程,但在作用方式及与底物的亲和力方面存在差异.
위연구분양화매(PO)재곤충세피과정중적공능화작용,채용미량측정법연구료황분충Tenebrio molitor체벽경화과정중혈림파화표피중적PO활성변화.결과표명:초세피유충혈림파중PO활성교고,단수착체벽적불단흑화여경화,기활성정현하강추세,재3～4 h내체도최저점,이후PO활성축점상승,7 h좌우활성상승지최고,병접근우정상유충적수평;재강세완피후적1 h내,체벽중 PO활성기본무변화,단수후즉개시하강,3 h좌우강도최저점,연후개시회승,6～7 h좌우회복도정상수평,병추우은정;이L-DOPA위저물,통과쌍도수곡선작도법구득황분충혈림파PO적K_m＝1.176 mmol/L,체벽PO적K_m＝0.881 mmol/L,표명체벽PO여저물L-DOPA적친화력요고우혈림파PO.연구표명량충래원적분양화매균삼여료황분충유충적체벽경화과정,단재작용방식급여저물적친화력방면존재차이.
The activity of phenoloxidase (PO) from the hemolymph and cuticle of Tenebrio molitor larvae was investigated at different time (1 -7 h) after molting with the microtitration method. The results showed that the hemolymph PO activity slowly decreased within 3 h after the larva molted but increased at 4 - 7 h after molting. The cuticle PO activity showed no remarkable changes within 1 h after the larva molted, but decreased at 3 h after molting. The PO activity nearly reached the normal level at 6 h after the larva molted. Meanwhile, determined by Lineweaver-Burk plot the K_m value of PO extracted from the hemolymph and cuticle was 1. 176 and 0. 881 mmol/L, respectively, indicating that PO from the cuticle has a higher affinity to substrate than that from the hemolymph. The results showed that both cuticle and hemolymph PO were involved in cuticle sclerotization of T. molitor, but have differences in action mode and affinity to substrate.