农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2008年
5期
47-49,62
,共4页
江芹%董玲%宁志怨%陈静娴%廖华俊
江芹%董玲%寧誌怨%陳靜嫻%廖華俊
강근%동령%저지원%진정한%료화준
Thesium chinense Turcz%Clustered shoots%Rooting
[Objective] The aim of this study is to establish the rapid micro-propagation system in Thesium chinense Turcz. [Method] With stem frag-ments of wild Thesium chinense Turcz as explants, different culture media were designed to conduct induction culture, strengthening plantlet culture and in vitro rooting. [Result] The optimum medium for inducing clustered shoots was determined to be MS medium appended with 1.5 mg/L 6-BA, 0. 01 mg/L NAA and 0.3 mg/L 2,4-D; in addition, 60 mg/kg ABT was suitable for rooting, by which the percentage of rooted plantlets reached 76.6%. [Conclu-sion] This study simplified the procedures of tissue culture in Thesium chinense Turcz and enhanced the proliferation rate, providing basis for artificial cul-tivation and resource protection of Thesium chinense Turcz.
