CAJ | 학술논문

为了明确禽大肠埃希菌的耐药情况和氨基糖苷抗性基因的流行情况,用K-B纸片扩散法检测了27株致病性大肠杆菌对19种常用抗生素的耐药谱,用PCR方法检测了耐氨基糖苷抗生素分离株的4种氨基糖苷抗性基因,包括腺苷转移酶基因aadA1和aadB、乙酰转移酶基因aacA4 和磷酸转移酶基因apH(3')-Ⅱ.结果表明,氨苄青霉素、安灭菌、青霉素G和四环素的耐药菌株率高达100%,利福平的耐药菌株率达96.3%,氯霉素、红霉素的耐药菌株为70.4%,卡那霉素、环丙沙星、氟哌酸的耐药菌株率为59.3%, 链霉素的耐药菌株达到63.0%.氨苄西林/舒巴坦复方制剂、复达欣、庆大霉素和磷霉素的高敏菌株率分别为100%、 77. 8%、74.1%和51.9%.氨基糖苷抗性基因aadA1、aacA4 和apH(3')-Ⅱ的阳性率分别是44.4%、27.8%和55.6%,aadB未捡出.这些数据提示禽致病性大肠埃希菌存在广泛的耐药谱,氨基糖苷抗性基因aadA1和apH-(3')-Ⅱ是主要流行基因.
위료명학금대장애희균적내약정황화안기당감항성기인적류행정황,용K-B지편확산법검측료27주치병성대장간균대19충상용항생소적내약보,용PCR방법검측료내안기당감항생소분리주적4충안기당감항성기인,포괄선감전이매기인aadA1화aadB、을선전이매기인aacA4 화린산전이매기인apH(3')-Ⅱ.결과표명,안변청매소、안멸균、청매소G화사배소적내약균주솔고체100%,리복평적내약균주솔체96.3%,록매소、홍매소적내약균주위70.4%,잡나매소、배병사성、불고산적내약균주솔위59.3%, 련매소적내약균주체도63.0%.안변서림/서파탄복방제제、복체흔、경대매소화린매소적고민균주솔분별위100%、 77. 8%、74.1%화51.9%.안기당감항성기인aadA1、aacA4 화apH(3')-Ⅱ적양성솔분별시44.4%、27.8%화55.6%,aadB미검출.저사수거제시금치병성대장애희균존재엄범적내약보,안기당감항성기인aadA1화apH-(3')-Ⅱ시주요류행기인.
In order to investigate the patterns of antibiotic resistance and prevalence of aminoglycoside resistant genes in pathogenic E.coli isolated from poultry, K-B disk diffusion method was used to detect the susceptibility of 27 E.coli isolates to 19 kinds of antimicrobial agents, and 4 aminoglycoside resistant genes of aminoglycoside adenyltransferases aadA1 and aadB,aminoglycoside phosphorylases apH(3')-Ⅱ and aminoglycoside acetyltransferases aacA4 were detected by using PCR. Results indicated that the resistance strains of 27 isolates to ampicillin,amoxicillin,penicillin G and tetracin were 100%, and that to rifampicin,chloromycin,erythromycin,Kanamycin,ciprofloxacin,norfloxacin and Streptomycin were 96.3%, 70.4%, 70.4%, 59.26%, 59.3%, 59.3%, and 63.0%, respectively. The percentage of sensitive E.coli isolates to Ampicillin/sulbactan,Fortum,Gentamicin and Fosfomycin were 100%, 77.8%, 74.1% and 51.9%, respectively. PCR results showed that 44.4%,27.8% and 55.6% of the tested isolates were positive for aadA1,aacA4 and apH(3')-Ⅱ,respectively. The aadB gene was not detected. These data showed that the spectrum of drug-resistance of avian pathogenic E.coli was very broad, and the aminoglycoside resistance gene of apH(3')-Ⅱ and aadA1 were leading prevalence genes.