林业科学
林業科學
임업과학
SCIENTIA SILVAE SINICAE
2009年
10期
94-99
,共6页
麦苗苗%石大兴%王米力%李伟
麥苗苗%石大興%王米力%李偉
맥묘묘%석대흥%왕미력%리위
连香树%PEG%种子引发%干旱胁迫%种子萌发%芽苗生长
連香樹%PEG%種子引髮%榦旱脅迫%種子萌髮%芽苗生長
련향수%PEG%충자인발%간한협박%충자맹발%아묘생장
Cercidiphyllum japonkum%PEG%seed priming%drought stress%seed germination%seedling growth
以连香树种子为试验材料,研究不同质量浓度PEG对种子萌发的影响.结果表明:适宜质量浓度(140～301 g·L~(-1))的PEG预处理连香树种子,能促进种子萌发和芽苗生长,增加渗透凋节物质如可溶性蛋白、可溶性糖和脯氨酸的含量,提高保护性酶SOD,POD和CAT的活性,降低MDA含量,修复膜系统损伤,提高其抗旱性.在干旱胁迫下,伴随PEG质量浓度的增加,连香树种子萌发和幼苗生长受到抑制,可溶性蛋白含量先升高后降低,可溶性糖、脯氨酸和MDA含量升高,保护性酶SOD和CAT的活性升高及POD活性下降.连香树对干旱胁迫较为敏感,PEG质量浓度≥95 g·L~(-1)对种子萌发、芽苗胚轴和胚根生长产生抑制作用.
以連香樹種子為試驗材料,研究不同質量濃度PEG對種子萌髮的影響.結果錶明:適宜質量濃度(140～301 g·L~(-1))的PEG預處理連香樹種子,能促進種子萌髮和芽苗生長,增加滲透凋節物質如可溶性蛋白、可溶性糖和脯氨痠的含量,提高保護性酶SOD,POD和CAT的活性,降低MDA含量,脩複膜繫統損傷,提高其抗旱性.在榦旱脅迫下,伴隨PEG質量濃度的增加,連香樹種子萌髮和幼苗生長受到抑製,可溶性蛋白含量先升高後降低,可溶性糖、脯氨痠和MDA含量升高,保護性酶SOD和CAT的活性升高及POD活性下降.連香樹對榦旱脅迫較為敏感,PEG質量濃度≥95 g·L~(-1)對種子萌髮、芽苗胚軸和胚根生長產生抑製作用.
이련향수충자위시험재료,연구불동질량농도PEG대충자맹발적영향.결과표명:괄의질량농도(140～301 g·L~(-1))적PEG예처리련향수충자,능촉진충자맹발화아묘생장,증가삼투조절물질여가용성단백、가용성당화포안산적함량,제고보호성매SOD,POD화CAT적활성,강저MDA함량,수복막계통손상,제고기항한성.재간한협박하,반수PEG질량농도적증가,련향수충자맹발화유묘생장수도억제,가용성단백함량선승고후강저,가용성당、포안산화MDA함량승고,보호성매SOD화CAT적활성승고급POD활성하강.련향수대간한협박교위민감,PEG질량농도≥95 g·L~(-1)대충자맹발、아묘배축화배근생장산생억제작용.
The morphological, physiological and biochemical indexes associated with seed germination and seedling growth of Cercidiphyllum japonicum were measured under treatments of seven different polyethylene glycol ( PEG) mass concentrations from 95 to 345 g·L~(-1). The results showed that the suitable PEG concentration was in a range from 140 to 301 g·L~(-1) . In this range, the membrane-repair capacity and the drought resistance of seeds were improved, accompanied with the changes of the following indicators. The contents of three osmoregulation substances [ soluble protein, sugar and proline) and the activities of three protective enzymes (superoxide dismutase (SOD) , peroxidase (POD) and catalase (CAT)] were increased, and the content of a final product of lipid peroxidation, malondialdehyde (MDA) was decreased. However, seed germination and seedling growth were inhibited in higher PEG mass concentrations under water stress. With increasing PEG mass concentrations, the contents of soluble sugar, proline and MDA, and the activities of two enzymes (SOD and CAT), were continuously raised, and the enzyme activity of POD was strongly inhibited. In addition, the content of soluble protein was increased first and then decreased in the condition. These results indicated that the seed germination and seedling growth were inhibited under the high PEG mass concentration (≥95 g·L~(-1)) . The results suggest that C. Japonicum seed is sensitive to drought stress.
