河南大学学报（自然科学版）
河南大學學報（自然科學版）
하남대학학보（자연과학판）
JOURNAL OF HENAN UNIVERSITY(NATURAL SCIENCE)
2009年
6期
621-625
,共5页
拟南芥%下旱突变体%TAIL-PCR技术%NCED3基因
擬南芥%下旱突變體%TAIL-PCR技術%NCED3基因
의남개%하한돌변체%TAIL-PCR기술%NCED3기인
Arabidopsis thaliana%drought mutant%TAIL-PCR method%NCED3 gene
利用远红外成像技术筛选得到一株T-DNA插入的干旱敏感突变体,并成功利用TAII,PCR技术克隆到该突变基因为NCED3,该基因编码植物体内ABA合成的关键限速酶,突变体在干旱胁迫下不能合成ABA而表现出不耐干旱的特性.最后通过PCR及RT PCR方法验证了TAIL-PCR结果的的靠性.
利用遠紅外成像技術篩選得到一株T-DNA插入的榦旱敏感突變體,併成功利用TAII,PCR技術剋隆到該突變基因為NCED3,該基因編碼植物體內ABA閤成的關鍵限速酶,突變體在榦旱脅迫下不能閤成ABA而錶現齣不耐榦旱的特性.最後通過PCR及RT PCR方法驗證瞭TAIL-PCR結果的的靠性.
이용원홍외성상기술사선득도일주T-DNA삽입적간한민감돌변체,병성공이용TAII,PCR기술극륭도해돌변기인위NCED3,해기인편마식물체내ABA합성적관건한속매,돌변체재간한협박하불능합성ABA이표현출불내간한적특성.최후통과PCR급RT PCR방법험증료TAIL-PCR결과적적고성.
By using infrared thermal imaging, we screened a drought-sensitive mutant and cloned this mutant gene NCED_3 by TAIL-PCR method, which is considered to be the rate-limiting step in abscisic acid (ABA) biosynthesis. The mutant line could not produc ABA under drought stress and showed decreased drought-tolerance phenotype. Subsequently, PCR and RT-PCR methods were used to confirm the authenticity of TAIL-PCR result.
