植物保护
植物保護
식물보호
PLANT PROTECTION
2009年
6期
127-129
,共3页
沈建国%王念武%翁瑞泉%黄可辉%郭琼霞
瀋建國%王唸武%翁瑞泉%黃可輝%郭瓊霞
침건국%왕념무%옹서천%황가휘%곽경하
菜豆荚斑驳病毒%检测%DAS-ELISA%IC-RT-PCR
菜豆莢斑駁病毒%檢測%DAS-ELISA%IC-RT-PCR
채두협반박병독%검측%DAS-ELISA%IC-RT-PCR
Bean pod mottle virus%detection%DAS-ELISA%IC-RT-PCR
本研究采用DAS-ELISA、IC-RT-PCR及序列测定方法,对加拿大进境大豆种子进行菜豆荚斑驳病毒(Bean pod mottle virus,BPMV)和大豆花叶病毒(Soybean mosaic virus,SMV)检测,结果表明,BPMV的DAS-ELISA和IC-RT-PCR检测结果均为阳性,且PCR扩增产物序列与已报道的BPMV基因序列相似性达97%以上,而SMV的DAS-ELISA和IC-RT-PCR检测结果都为阴性.综合血清学、分子生物学检测结果,确认该批大豆携带有菜豆荚斑驳病毒.
本研究採用DAS-ELISA、IC-RT-PCR及序列測定方法,對加拿大進境大豆種子進行菜豆莢斑駁病毒(Bean pod mottle virus,BPMV)和大豆花葉病毒(Soybean mosaic virus,SMV)檢測,結果錶明,BPMV的DAS-ELISA和IC-RT-PCR檢測結果均為暘性,且PCR擴增產物序列與已報道的BPMV基因序列相似性達97%以上,而SMV的DAS-ELISA和IC-RT-PCR檢測結果都為陰性.綜閤血清學、分子生物學檢測結果,確認該批大豆攜帶有菜豆莢斑駁病毒.
본연구채용DAS-ELISA、IC-RT-PCR급서렬측정방법,대가나대진경대두충자진행채두협반박병독(Bean pod mottle virus,BPMV)화대두화협병독(Soybean mosaic virus,SMV)검측,결과표명,BPMV적DAS-ELISA화IC-RT-PCR검측결과균위양성,차PCR확증산물서렬여이보도적BPMV기인서렬상사성체97%이상,이SMV적DAS-ELISA화IC-RT-PCR검측결과도위음성.종합혈청학、분자생물학검측결과,학인해비대두휴대유채두협반박병독.
The presence of BPMV and SMV in soybean seeds imported from Camda was determined using DASELISA,IC-RT-PCR and sequence analysis.The results showed that both DAS-ELISA and IC-RT-PCR were positive for BPMV,and the nucleotide sequence of BPMV PCR product had more than 97% similarity to previously reported genornic sequence.However,negative DAS-ELISA and IC-RT-PCR results were obtained for SMV.According to the results of serological and molecular biological methods,it was confirmed that BPMV was present in tested soybean imported from Camda.
