中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2005年
33期
166-168
,共3页
高庆春%邵延霞%易咏红%陈颖贤%黄如训
高慶春%邵延霞%易詠紅%陳穎賢%黃如訓
고경춘%소연하%역영홍%진영현%황여훈
高血压%血压%脑血管损伤%大鼠
高血壓%血壓%腦血管損傷%大鼠
고혈압%혈압%뇌혈관손상%대서
背景:近来提出的临界关闭压在脑血流自动调节中起着关键作用,是脑循环的有效下游压,可客观反映动态调节的脑血管紧张度,即血管平滑肌收缩舒张的功能状态.目的:利用临界关闭压动态评价高血压对脑微动脉平滑肌收缩功能的损伤及其与病理形态改变间的关系.设计:随机对照的实验. 单位:广州医学院第二附属医院神经科学研究所、中山大学附属第一医院神经科.材料:实验于2002-07/2003-08在中山大学生理科学实验室进行.选择健康雄性SD大鼠160只,随机分为对照组和高血压组,每组80只.方法:高血压组用双肾双夹法复制成易卒中型肾血管性高血压大鼠模型.对照组除不上银夹外,其余处理同高血压组.两组动物在术后的第2,4,6,8,10,12,14及16周8个时间点各随机选取10只进行动脉血压、临界关闭压的测定.测定完毕后在麻醉状态下取大鼠额顶叶脑组织,制成切片,定量分析脑微动脉的形态变化.主要观察指标:①两组大鼠术后不同时间点动脉血压、临界关闭压.②两组大鼠术后不同时间点脑微动脉形态参数.结果:160只大鼠均进入结果分析.①高血压组平均动脉压于术后第6周起明显升高,均显著高于对照组(P＜0.05).②高血压组大鼠术后脑血管临界关闭压逐步升高,于术后14,16周明显高于对照组[(63.75±7.43),(37.28±3.68)mm Hg;(67.37±15.57),(38.39±7.41)mm Hg,P＜0.05].③高血压组大鼠脑内微动脉的中膜厚度增加非常明显,于第8周后显著大于对照组(P＜0.05).④临界关闭压的升高与平均动脉压、脑内微动脉的中膜厚度呈正相关(r=0.906 93,0.811 36,P均＜0.05).并且血压升高的初期和血压较高后临界关闭压变化较明显,于血压上升明显期则改变不显著,呈倒"S"形改变(R2=0.996 2,P＜0.05).结论:高血压形成后脑微动脉平滑肌的收缩程度随动脉血压的动态升高而加强,血管紧张度相应提高,并且在高血压初期和血压较高后改变比较明显.
揹景:近來提齣的臨界關閉壓在腦血流自動調節中起著關鍵作用,是腦循環的有效下遊壓,可客觀反映動態調節的腦血管緊張度,即血管平滑肌收縮舒張的功能狀態.目的:利用臨界關閉壓動態評價高血壓對腦微動脈平滑肌收縮功能的損傷及其與病理形態改變間的關繫.設計:隨機對照的實驗. 單位:廣州醫學院第二附屬醫院神經科學研究所、中山大學附屬第一醫院神經科.材料:實驗于2002-07/2003-08在中山大學生理科學實驗室進行.選擇健康雄性SD大鼠160隻,隨機分為對照組和高血壓組,每組80隻.方法:高血壓組用雙腎雙夾法複製成易卒中型腎血管性高血壓大鼠模型.對照組除不上銀夾外,其餘處理同高血壓組.兩組動物在術後的第2,4,6,8,10,12,14及16週8箇時間點各隨機選取10隻進行動脈血壓、臨界關閉壓的測定.測定完畢後在痳醉狀態下取大鼠額頂葉腦組織,製成切片,定量分析腦微動脈的形態變化.主要觀察指標:①兩組大鼠術後不同時間點動脈血壓、臨界關閉壓.②兩組大鼠術後不同時間點腦微動脈形態參數.結果:160隻大鼠均進入結果分析.①高血壓組平均動脈壓于術後第6週起明顯升高,均顯著高于對照組(P＜0.05).②高血壓組大鼠術後腦血管臨界關閉壓逐步升高,于術後14,16週明顯高于對照組[(63.75±7.43),(37.28±3.68)mm Hg;(67.37±15.57),(38.39±7.41)mm Hg,P＜0.05].③高血壓組大鼠腦內微動脈的中膜厚度增加非常明顯,于第8週後顯著大于對照組(P＜0.05).④臨界關閉壓的升高與平均動脈壓、腦內微動脈的中膜厚度呈正相關(r=0.906 93,0.811 36,P均＜0.05).併且血壓升高的初期和血壓較高後臨界關閉壓變化較明顯,于血壓上升明顯期則改變不顯著,呈倒"S"形改變(R2=0.996 2,P＜0.05).結論:高血壓形成後腦微動脈平滑肌的收縮程度隨動脈血壓的動態升高而加彊,血管緊張度相應提高,併且在高血壓初期和血壓較高後改變比較明顯.
배경:근래제출적림계관폐압재뇌혈류자동조절중기착관건작용,시뇌순배적유효하유압,가객관반영동태조절적뇌혈관긴장도,즉혈관평활기수축서장적공능상태.목적:이용림계관폐압동태평개고혈압대뇌미동맥평활기수축공능적손상급기여병리형태개변간적관계.설계:수궤대조적실험. 단위:엄주의학원제이부속의원신경과학연구소、중산대학부속제일의원신경과.재료:실험우2002-07/2003-08재중산대학생이과학실험실진행.선택건강웅성SD대서160지,수궤분위대조조화고혈압조,매조80지.방법:고혈압조용쌍신쌍협법복제성역졸중형신혈관성고혈압대서모형.대조조제불상은협외,기여처리동고혈압조.량조동물재술후적제2,4,6,8,10,12,14급16주8개시간점각수궤선취10지진행동맥혈압、림계관폐압적측정.측정완필후재마취상태하취대서액정협뇌조직,제성절편,정량분석뇌미동맥적형태변화.주요관찰지표:①량조대서술후불동시간점동맥혈압、림계관폐압.②량조대서술후불동시간점뇌미동맥형태삼수.결과:160지대서균진입결과분석.①고혈압조평균동맥압우술후제6주기명현승고,균현저고우대조조(P＜0.05).②고혈압조대서술후뇌혈관림계관폐압축보승고,우술후14,16주명현고우대조조[(63.75±7.43),(37.28±3.68)mm Hg;(67.37±15.57),(38.39±7.41)mm Hg,P＜0.05].③고혈압조대서뇌내미동맥적중막후도증가비상명현,우제8주후현저대우대조조(P＜0.05).④림계관폐압적승고여평균동맥압、뇌내미동맥적중막후도정정상관(r=0.906 93,0.811 36,P균＜0.05).병차혈압승고적초기화혈압교고후림계관폐압변화교명현,우혈압상승명현기칙개변불현저,정도"S"형개변(R2=0.996 2,P＜0.05).결론:고혈압형성후뇌미동맥평활기적수축정도수동맥혈압적동태승고이가강,혈관긴장도상응제고,병차재고혈압초기화혈압교고후개변비교명현.
BACKGROUND: Critical closing pressure (CCP) is recently thought to play a key role in cerebral blood flow autoregulation as an effective downstream pressure of cerebral circulation and can objectively reflect the cerebrovascular tone, namely the vascular smooth muscle contraction and diastole, which is subjected to dynamic modulation.OBJECTIVE: To dynamically assess the hypertension-induced damage of the contraction function of cerebral microvascular smooth muscles and its correlation with morphological changes based on CCP evaluation.DESIGN: Randomized controlled experiment.SETTING: Institute of Neural Science of Second Hospital Affiliated to Guangzhou Medical College and Department of Neurology, First Hospital Affiliated to Sun Yet-san University.MATERIALS: The experiment was carried out at the Laboratory of Physiological Science of Sun Yet-san University between July 2002 and August 2003. Totally 160 health male SD rats were randomized into control group and hypertension group with 80 rats in each group. METHODS: Stroke-prone renovas cular hyp ortonsive rats were established in rats of the hypertension group by bilateral renal artery occlusion with two clips. The rats in the control group were not subjected to the occlusion with other treatments identical to those of the hypertension group. At the time points of 2, 4, 6, 8, 10, 12, 14 and 16 weeks after operation, respectively, 10 rats were randomly selected from each of the two groups for determination of arterial pressure and CCP. After the measurements the frontal-parietal lobe was obtained from the anaesthetized rats and cut into slices for quantitative analysis of the morphological changes in cerebral microvessels.different postoperative time points.mean arterial pressure in hypertension group obviously increased from the 6th postoperative week with significant difference from that of the control after operation to a level significantly higher than that of the control group at postoperative 14 and 16 weeks [(63.75±7.43) vs (37.28±3.68) mm Hg and (67.37±15.57) vs (38.39t7.41) mm Hg, respectively, P ＜ 0.05].significance from that of the control group at the 8th postoperative week (Paverage arterial pressure and cerebral arteriole tunica media (r=0.906 93,0.811 36, respectively, P ＜ 0.05). The changes in CCP was more obvious in the early and advanced stages of blood pressure elevation, but not so manifest during obvious blood pressure increment, displaying an inverted S-shaped curve of changes (R2=0.996 2, P ＜ 0.05).CONCLUSION: Contraction of the cerebrovascular smooth muscles is enhanced with the dynamic increment of arterial pressure after the development of hypertension. Vascular tone increase is more manifest during the early and advanced stages of hypertension.
