CAJ | 학술논문

目的探讨高表达热休克蛋白A12B(HSPA12B)对内毒素血症所致心功能不全的影响.方法 (1) 采用western blot法鉴定HSPA12B在HSPA12B转基因(HSPA12B Tg)鼠心脏中的表达;(2) HSPA12B Tg和野生型(WT)对照鼠均腹腔注射内毒素(LPS)10 mg/kg,6 h后以超声心动图测定心功能;(3) LPS注射后6 h,收集心脏,石蜡切片,HE染色,分析组织水肿和白细胞浸润程度.结果 (1) HSPA12B Tg鼠心肌组织中HSPA12B表达较WT鼠升高12倍(P＜0.01);(2) LPS使WT和HSPA12B Tg鼠心功能均显著下降,但与WT鼠比较,HSPA12B Tg鼠的心功能显著改善(P＜0.01),射血分数HEF)增加了56.0%,缩短分数HFS)增加了72.5%;(3) LPS使WT和HSPA12B Tg鼠心肌间质中白细胞浸润,但与WT鼠比较,HSPA12B Tg鼠的心肌间质中白细胞浸润数量显著减少45.1%(P＜0.05).结论 HSPA12B高表达显著抑制内毒素血症所致的心功能不全、减轻白细胞在心肌间质的浸润程度.
목적 탐토고표체열휴극단백A12B(HSPA12B)대내독소혈증소치심공능불전적영향.방법 (1) 채용western blot법감정HSPA12B재HSPA12B전기인(HSPA12B Tg)서심장중적표체;(2) HSPA12B Tg화야생형(WT)대조서균복강주사내독소(LPS)10 mg/kg,6 h후이초성심동도측정심공능;(3) LPS주사후6 h,수집심장,석사절편,HE염색,분석조직수종화백세포침윤정도.결과 (1) HSPA12B Tg서심기조직중HSPA12B표체교WT서승고12배(P＜0.01);(2) LPS사WT화HSPA12B Tg서심공능균현저하강,단여WT서비교,HSPA12B Tg서적심공능현저개선(P＜0.01),사혈분수HEF)증가료56.0%,축단분수HFS)증가료72.5%;(3) LPS사WT화HSPA12B Tg서심기간질중백세포침윤,단여WT서비교,HSPA12B Tg서적심기간질중백세포침윤수량현저감소45.1%(P＜0.05).결론 HSPA12B고표체현저억제내독소혈증소치적심공능불전、감경백세포재심기간질적침윤정도.