CAJ | 학술논문

建立了鉴别体液中三唑仑及其主要代谢物的液相色谱-质谱联用法。采用液相色谱-电喷雾离子阱质谱法，得到三唑仑和羟基化代谢物及其葡萄糖苷酸型结合物的色谱、质谱信息以及特征碎片离子。三唑仑的最低检测限小于0.1ng。用本法分析了一例怀疑服用过量三唑仑的麻醉抢劫案例所取证的血样及尿样，并与健康受试者服药后尿样及家兔灌胃三唑仑后所取的血样及尿样比较，得到了当事人服用三唑仑的可靠数据。该方法可靠、快速、简便，尤其适用于需快速响应的法医学和毒理学分析。
건립료감별체액중삼서륜급기주요대사물적액상색보-질보련용법。채용액상색보-전분무리자정질보법，득도삼서륜화간기화대사물급기포도당감산형결합물적색보、질보신식이급특정쇄편리자。삼서륜적최저검측한소우0.1ng。용본법분석료일례부의복용과량삼서륜적마취창겁안례소취증적혈양급뇨양，병여건강수시자복약후뇨양급가토관위삼서륜후소취적혈양급뇨양비교，득도료당사인복용삼서륜적가고수거。해방법가고、쾌속、간편，우기괄용우수쾌속향응적법의학화독이학분석。
A highly sensitive method was described for the detection oftriazolam and its major metabolites, α-hydroxytriazolam and 4-hydroxytriazolam, in the plasma and urine with liquid chromatography-mass spectrometry system which incorporated an electrospray ionization interface. After oral administration of a single 0.25 mg dose of triazolam to a healthy volunteer as well as a single 40 mg/kg dose to a rabbit,the plasma and urine samples were extracted and then injected into an ODS column with a mobile phase of 80% methanol and 20% water for LC/MS analysis. The structures of triazolam and its metabolites were identified either by direct comparison of the observed mass spectra and the chromatographic retention time with reference spectra or by the characteristic ions in the selected-ion monitoring as well as full scan MS2 (or MS3) mode. The detection limit was below 0.1 ng of triazolam. By this method, we demonstrated the presence of triazolam in the unknown plasma and urine samples from a human being. It is proved that this method is especially suitable for the detection in body fluids of forensical and clinical toxicological relevant triazolam.