CAJ | 학술논문

以中国对虾为实验材料，研究影响对虾淋巴组织原代培养的一些因素。研究中发现，用75%的乙醇浸泡对虾就可以达到淋巴组织培养无菌的要求，当组织块的接种密度为240～260块(25 cm2)-1时最适。当原代细胞培养30d左右，细胞会因接触抑制而停止生长，当接触抑制消除后，细胞仍可继续生长。另外，还进行了以N-甲基-N′-亚硝基胍（MNNG）对原代细胞进行诱变的尝试，发现诱变后有的细胞呈现出转化细胞的形态，并形成了转化灶。
이중국대하위실험재료，연구영향대하림파조직원대배양적일사인소。연구중발현，용75%적을순침포대하취가이체도림파조직배양무균적요구，당조직괴적접충밀도위240～260괴(25 cm2)-1시최괄。당원대세포배양30d좌우，세포회인접촉억제이정지생장，당접촉억제소제후，세포잉가계속생장。령외，환진행료이N-갑기-N′-아초기고（MNNG）대원대세포진행유변적상시，발현유변후유적세포정현출전화세포적형태，병형성료전화조。
Some factors affecting the primary culture of Penaeus chinensis lymphatic cells were studied. It was found that simply rinsing the shrimp in 75% ethanol could obtain satisfactory result in sterilization, the optimal inoculating density was 240～260 blocks/25cm2. After cultured for about 30 days, the cells stopped growing due to contact inhibition. However, after the inhibition was broken by scratching away some cells, the cells continued to grow. Moreover, after we had tried to mutagenize the cells with N-methyl-N｀-nitrosoguanidine (MNNG), some transformed cells had some morphological changes and grew in multilayer. In the meantime, many foci turned up.