高血压杂志
高血壓雜誌
고혈압잡지
2001年
2期
140-142
,共3页
高血压%一肾一夹%大鼠%动脉平滑肌细胞%钠泵%基因表达
高血壓%一腎一夾%大鼠%動脈平滑肌細胞%鈉泵%基因錶達
고혈압%일신일협%대서%동맥평활기세포%납빙%기인표체
目的 探讨“一肾一夹(1k1c)”高血压大鼠动脉平滑肌细胞钠泵α亚单位各异构体基 因表达的改变，为高血压发病机制的深入研究提供理论及实验依据。方法 分别应用分子生物学 RT-PCR及免疫组化技术，探讨1k1c高血压大鼠动脉平滑肌细胞钠泵α1、α2及α3亚单位mR NA及蛋白 水平基因表达的改变。结果 1k1c高血压大鼠动脉平滑肌细胞钠泵α亚单位基因表达发生改变： 无论在mRNA或蛋白水平，α1亚单位表达增强，而α2与α3亚单位表达无改变。结论 1k1c高血压大鼠动脉平滑肌细胞钠泵α亚单位基因表达发生改变，这种 改变可能参与了该模型的血压升高 机制。
目的 探討“一腎一夾(1k1c)”高血壓大鼠動脈平滑肌細胞鈉泵α亞單位各異構體基 因錶達的改變，為高血壓髮病機製的深入研究提供理論及實驗依據。方法 分彆應用分子生物學 RT-PCR及免疫組化技術，探討1k1c高血壓大鼠動脈平滑肌細胞鈉泵α1、α2及α3亞單位mR NA及蛋白 水平基因錶達的改變。結果 1k1c高血壓大鼠動脈平滑肌細胞鈉泵α亞單位基因錶達髮生改變： 無論在mRNA或蛋白水平，α1亞單位錶達增彊，而α2與α3亞單位錶達無改變。結論 1k1c高血壓大鼠動脈平滑肌細胞鈉泵α亞單位基因錶達髮生改變，這種 改變可能參與瞭該模型的血壓升高 機製。
목적 탐토“일신일협(1k1c)”고혈압대서동맥평활기세포납빙α아단위각이구체기 인표체적개변，위고혈압발병궤제적심입연구제공이론급실험의거。방법 분별응용분자생물학 RT-PCR급면역조화기술，탐토1k1c고혈압대서동맥평활기세포납빙α1、α2급α3아단위mR NA급단백 수평기인표체적개변。결과 1k1c고혈압대서동맥평활기세포납빙α아단위기인표체발생개변： 무론재mRNA혹단백수평，α1아단위표체증강，이α2여α3아단위표체무개변。결론 1k1c고혈압대서동맥평활기세포납빙α아단위기인표체발생개변，저충 개변가능삼여료해모형적혈압승고 궤제。
Objective To investigate the gene expression of sodium pump α-subunit in aortic smooth muscle of 1-kidney-1-clip (1k1c) hypertensive rats. Methods 1k1c hypertensive rats were prepared by partially ligating the left renal artery and removing the right kidney. 4 weeks later, all the rats were killed and sodium pump α1- , α2-, and α3-subunit in aortic smooth muscles were detected with reverse transcription p ol ymerase chain reaction (RT-PCR) method and immunohistochemical assay at both mR NA and protein levels, respectively. Resulsts Changes in the expression of sodi um pump α-subunit gene were found in aortic smooth muscles of 1k1c hypertensiv e rats: α1-su bunit increased at both mRNA protein levels, while α2- and α3-subunits r emained without changes. Conclusions There were great changes in the gene expression of so dium pump α-subunit in aor tic smooth muscles of 1k1c hypertensive rats, which might be related to the development of hypertension in this hypertensive model.
