CAJ | 학술논문

目的确定鼠脑细胞体外培养的适宜生长基质。方法以Balb/C鼠尾胶原与大鼠尾胶原作为生长基质，培养新生Balb/C鼠脑细胞。结果 Balb/C鼠尾胶原有利于神经元的生存、生长与分化，而大鼠尾胶原则利于星形胶质细胞的生存、生长、分化与增殖。结论 2种鼠尾胶原均为培养鼠脑细胞适宜的生长基质，培养1个月左右宜用Balb/C鼠尾胶原而长期培养则宜选择大鼠尾胶原。
목적학정서뇌세포체외배양적괄의생장기질。방법이Balb/C서미효원여대서미효원작위생장기질，배양신생Balb/C서뇌세포。결과 Balb/C서미효원유리우신경원적생존、생장여분화，이대서미효원칙리우성형효질세포적생존、생장、분화여증식。결론 2충서미효원균위배양서뇌세포괄의적생장기질，배양1개월좌우의용Balb/C서미효원이장기배양칙의선택대서미효원。
Objective　To determine the suitable growth substrate for brain cells in culture. Methods　Two kinds of collagen from Balb/C mouse tail and rat tail respectively as growth substrate was applied to culturing newborn Balb/C mouse brain cells. Results　Balb/C mouse tail collagen was suitable to the survival, growth, differentiation of neurons, whereas rat tail collagen those of astrocytes. Conclusion　Two kinds of collagen are suitable as growth substrate of neuron culture. Balb/C mouse tail collagen may be applied as growth substrate for short-term cultures, whereas rat tail collagen may be adopted for long-term cultures.