CAJ | 학술논문

组织因子途径抑制因子(tissue factor pathway in hibitor, TFPI)是组织因子凝血途径的主要抑制因子，具有抑制组织因子、凝血因子VIIa、和Xa的功能。我们以前的研究显示TFPI在体外可以明显延长被修饰材料表面的凝血时间，在体内显著减少材料表面的血栓形成。本文观察了TFPI包被对聚乙烯和聚氯乙烯材料表面血小板粘附的影响。结果显示，通过TFPI处理后，上述两种材料表面的血小板粘附数目较对照组明显减少，提示TFPI可通过抑制血小板在材料表面的粘附起到改善生物材料血液相容性的作用。
조직인자도경억제인자(tissue factor pathway in hibitor, TFPI)시조직인자응혈도경적주요억제인자，구유억제조직인자、응혈인자VIIa、 화Xa적공능。아문이전적연구현시TFPI재체외가이명현연장피수식재료표면적응혈시간，재 체내현저감소재료표면적혈전형성。본문관찰료TFPI포피대취을희화취록을희재료표면혈소 판점부적영향。결과현시，통과TFPI처리후，상술량충재료표면적혈소판점부수목교대조조 명현감소，제시TFPI가통과억제혈소판재재료표면적점부기도개선생물재료혈액상용성적작용。
Tissue factor pathway inhibitor(TFPI) is the majo rinhibitor of the extrinsic pathway of the blood coagulation cascade. Our previous study showed that TFPI could obviously prolong the blood coagulation time on the surface of polyethelene(PE) and polyvinylchloride(PVC) membranes in vitro and decrease the generation of thrombus on Dacron membrane in vivo. In the pressent sutdy, the effect of TFPI on the adhesion of platelet onto PE and PVC membr anes was investigated. PE or PVC membrane was cut into small pieces and incubated in 96-well culture plate with GST or GST-TFPI fusion protein at 4℃ over night. Freshly collected rabbit blood was mixed with 3.8% sodium citrate. Platelet-rich plasma was separated by centrifugation and then incubated with the treated membranes at 37℃ for 30 minutes. The membranes were then washed, fixed, and freeze-dehydrated Adhesion of platelet was observed through scanning electronic microscope. The result showed that GST-TFPI treatment significantly decrea sed the number of platelet adhered on the membrane when compared with the GST and control group,and it suggested that TFPI might have a great potential to be used as an anticoagulant for improving the hemocompatibility of biomaterials.