CAJ | 학술논문

利用蛋白酶的溶蛋白特性，结合琼脂免疫扩散试验，建立了脱脂奶溶蛋白琼脂扩散抑制试验的新方法，用以检测兔血清中的嗜水气单胞菌(Aeromonas hyd rophilla)胞外蛋白酶抗体。同时与琼脂扩散免疫沉淀试验及ELISA试验进行了比较，结果显示，该方法的敏感性优于琼脂扩散免疫沉淀试验，而与ELISA试验相当。
이용단백매적용단백특성，결합경지면역확산시험，건립료탈지내용단백경지확산억제시험적신방법，용이검측토혈청중적기수기단포균(Aeromonas hyd rophilla)포외단백매항체。동시여경지확산면역침정시험급ELISA시험진행료비교，결과현시，해방법적민감성우우경지확산면역침정시험，이여ELISA시험상당。
A new method—skim milk proteolysis agar diffusion inhabit test was established according to the proteolysis character of proteases and the agar immunodiffusion test(AIDT).The new method was used to detect the antibody to extrocellular proteases of Aeromonas hydrophila from rabbit serum.And compared with the agar immunodiffusion test and the ELISA,it sh owed that the new method was more sensitive than the AIDT and equal as ELISA.