CAJ | 학술논문

以黄海黄杆菌YS-9412-130菌株为出发株，经亚硝基胍、硫酸二乙酯、紫外线(UV)与微波(MI)复合诱变和自然选育,获得1株产低温碱性蛋白酶高产稳定的突变株YS-9412-130-SW1-104，其产低温碱性蛋白酶为出发株的16倍。在摇瓶发酵培养条件下，酶活力达2　320U/ml（20℃测定），该诱变株具有Refr、Strr、Ampr和Met-、Lys-标记。突变株与出发菌株在细胞形态上比较，结果表明二者没有显著差异。
이황해황간균YS-9412-130균주위출발주，경아초기고、류산이을지、자외선(UV)여미파(MI)복합유변화자연선육,획득1주산저온감성단백매고산은정적돌변주YS-9412-130-SW1-104，기산저온감성단백매위출발주적16배。재요병발효배양조건하，매활력체2　320U/ml（20℃측정），해유변주구유Refr、Strr、Ampr화Met-、Lys-표기。돌변주여출발균주재세포형태상비교，결과표명이자몰유현저차이。
In screening high producer of low-temperature alkaline protease, F. yellowsea YS-9412-130 is chosen as original strain, which is treated repeatedly with various mutagens(NTG、DEM、UV and Microwave). The mutant YS-9412-130-SW1-104 with high and stable yield of alkaline protease is selected after repeated natural selection. The alkaline protease activity of this mutant is in the 16 fold of the original strain, reaching 2 320U/ml (assayed 20℃). The mutant has marked with Refr、Strr、Ampr 、Met- and Lys-.The result shows that there are no significant differences between the cells of the mutant and the original strain.