南京铁道医学院学报
南京鐵道醫學院學報
남경철도의학원학보
JOURNAL OF NANJING RAIL WAY MEDICAL COLLEGE
2000年
4期
234-237
,共4页
10-羟基喜树碱%人肝癌细胞%凋亡%p53基因
10-羥基喜樹堿%人肝癌細胞%凋亡%p53基因
10-간기희수감%인간암세포%조망%p53기인
10-hydroxycamptothecin%human hepatoma cells%apoptosis%p53 gene
目的：研究10-羟基喜树碱(10-HCPT)诱导人肝癌细胞凋亡的作用。方法：用10-HCPT以不同终浓度、不同作用时间诱导SMMC-721人肝癌细胞。分别以倒置相差显微镜、电镜、荧光显微镜观察其形态学改变，提取细胞内小分子DNA进行琼脂糖凝胶电泳，并对这些细胞的P53蛋白进行免疫组织化学染色。结果：当10-HCP终浓度>2.0μmol·L-1时，部分细胞可见典型的凋亡特征：核固缩、凋亡小体、梯状电泳带。随着10-HCPT浓度或作用时间增加，凋亡率逐渐升高。各组细胞P53蛋白免疫组化染色均为阴性。结论：10-HCPT可诱导SMMCC-7721细胞凋亡，其作用效果呈剂量、时间依赖性。
目的：研究10-羥基喜樹堿(10-HCPT)誘導人肝癌細胞凋亡的作用。方法：用10-HCPT以不同終濃度、不同作用時間誘導SMMC-721人肝癌細胞。分彆以倒置相差顯微鏡、電鏡、熒光顯微鏡觀察其形態學改變，提取細胞內小分子DNA進行瓊脂糖凝膠電泳，併對這些細胞的P53蛋白進行免疫組織化學染色。結果：噹10-HCP終濃度>2.0μmol·L-1時，部分細胞可見典型的凋亡特徵：覈固縮、凋亡小體、梯狀電泳帶。隨著10-HCPT濃度或作用時間增加，凋亡率逐漸升高。各組細胞P53蛋白免疫組化染色均為陰性。結論：10-HCPT可誘導SMMCC-7721細胞凋亡，其作用效果呈劑量、時間依賴性。
목적：연구10-간기희수감(10-HCPT)유도인간암세포조망적작용。방법：용10-HCPT이불동종농도、불동작용시간유도SMMC-721인간암세포。분별이도치상차현미경、전경、형광현미경관찰기형태학개변，제취세포내소분자DNA진행경지당응효전영，병대저사세포적P53단백진행면역조직화학염색。결과：당10-HCP종농도>2.0μmol·L-1시，부분세포가견전형적조망특정：핵고축、조망소체、제상전영대。수착10-HCPT농도혹작용시간증가，조망솔축점승고。각조세포P53단백면역조화염색균위음성。결론：10-HCPT가유도SMMCC-7721세포조망，기작용효과정제량、시간의뢰성。
Objective To investigate the effect of 10-hydroxycamptothecin(10-HCPT) on the induction of apeptosis in human hepatoma cells. Methods SMMC-7721 human hepatoma cells were treated with 10-HCPT in different concentration or for different time in vitro. Their morphological changes were detected by light microscopy, electron microscopy and fluorescence microscopy. In addition, low molecular-weight DNA was isolated fr om those cells, and then subjected to electtophoresis through a agarose gel. Furthermore, P53 protein in those cells was investigated by immunohistochemistry. Results When concentration of 10-HCPT was higher than 2.0 μmol· L- 1, the hallmarks of apoptosis were detected in some cells ( condensed chromatin,apoptosis bodies,“ladder” pattern of intemucleosomal DNA cleavage). The percentage of apoptotic cells rose with increasing concentration of 10-HCPT or prolonging exposure time. Immunohistochemistry staining for p53 protein was negative in both treated and untreated cells. Conclusion 10-HCPT can induce apoptosis in SMMC-7721 human hepatoma cells. The efficiency of 10-HCPT depended on both drug concentration and exposure time.
