CAJ | 학술논문

目的:研究Epstein-Barr病毒(EBV)与腮腺淋巴上皮瘤样癌(lymphoepithelioma-like carcinoma, LELC)的关系,并检测瘤细胞内EBV基因编码产物.方法:作者收集了中山医科大学所属病理科1986年1月至1995年12 月间32例腮腺LELCs..32例LELC石蜡包埋标本再次切片.采用免疫组化和原位核酸杂交法检测瘤细胞内EBV基因表达产物.结果:(1) 在125例腮腺癌中有32例淋巴上皮瘤样癌,占总病例的25.6%(32/125).(2)所有32例腮腺LELC组织中均有数量不等的EBNA-1和EBERs阳性瘤细胞.(3)27例LELC组织中部分瘤细胞表达LMP-1.(4)所有标本中均未见ZEBRA阳性细胞.(5)32例腮腺LELC组织中EA-D、VCA和MA的阳性表达率分别为71.9%(23/32)、68.8%(22/32)和12.5%(4/32).结论:(1)在鼻咽癌高发的广州地区,腮腺LELC的发病率也较高.(2)腮腺LELC组织中均有EB病毒感染.(3)EB病毒在腮腺LELC的感染主要为潜伏Ⅱ型,即表达EBNA-1、EBERs和LMP-1.(4)在腮腺LELC瘤细胞内潜伏感染的EB病毒可部分进入溶解周期,从而表达EA-D、VCA和MA.
목적:연구Epstein-Barr병독(EBV)여시선림파상피류양암(lymphoepithelioma-like carcinoma, LELC)적관계,병검측류세포내EBV기인편마산물.방법:작자수집료중산의과대학소속병이과1986년1월지1995년12 월간32례시선LELCs..32례LELC석사포매표본재차절편.채용면역조화화원위핵산잡교법검측류세포내EBV기인표체산물.결과:(1) 재125례시선암중유32례림파상피류양암,점총병례적25.6%(32/125).(2)소유32례시선LELC조직중균유수량불등적EBNA-1화EBERs양성류세포.(3)27례LELC조직중부분류세포표체LMP-1.(4)소유표본중균미견ZEBRA양성세포.(5)32례시선LELC조직중EA-D、VCA화MA적양성표체솔분별위71.9%(23/32)、68.8%(22/32)화12.5%(4/32).결론:(1)재비인암고발적엄주지구,시선LELC적발병솔야교고.(2)시선LELC조직중균유EB병독감염.(3)EB병독재시선LELC적감염주요위잠복Ⅱ형,즉표체EBNA-1、EBERs화LMP-1.(4)재시선LELC류세포내잠복감염적EB병독가부분진입용해주기,종이표체EA-D、VCA화MA.
Purpose To investigate the relationship between Epstein-Barr virus (EBV) and lymphoepithelioma-like carcinoma (LELC) of the parotid gland and detect the gene expression products of EBV harbouring in LELC cells. Methods Thirty-two parotid LELCs were collected from the Departments of Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou, China during the period of January 1986 and December 1995. All the 32 formalin-fixed paraffin-embedded blocks had been consecutively re-sectioned again. Immunohistochemical and in situ nucleic acid hybridization methods for detection of EBV gene encoded products were performed. Results (1) 32 LELCs were found out of 125 parotid gland carcinomas, the frequency was 25.6% (32/125). (2) All of the 32 specimens contained a variable number of EBNA-1 and EBERs positive neoplastic cells. (3) Twenty-seven out of 32 specimens (27/32, 84.4%) had a portion of carcinoma cells expressing LMP-1. (4) No ZEBRA positive cell could be found. (5) EA-D, VCA and MA positivity rates for these 32 parotid LELCs reached to 71.9%(23/32), 68.8%(22/32), and 12.5%(4/32), respectively. Conclusions (1) The parotid gland LELC is frequently to be seen in Guangzhou locale of China, where is a high-incidence area of nasopharyngeal carcinoma (NPC). The parotid gland LELC and NPC are co-prevalent in Guangzhou locale. (2) This disease is also consistently associated with EBV infection. (3) The EBV infection of the parotid gland LELCs is essentially the type of latency Ⅱ, expressing EBNA-1, EBERs and LMP-1. (4) The latent infected EBV harbouring in LELC cells could in part be switched over to lytic cycle, producing EA-D, VCA or/and MA.