CAJ | 학술논문

将牛泡沫病毒(BFV3026)感染的细胞经耳缘静脉注射兔子,并以正常细胞注射的兔为对照.1年后处死,病毒挽救实验及PCR检测显示:兔经一次注射即可被BFV3026感染,病毒广泛分布于感染兔的多种脏器中,通过共培养可从感染兔血、肝、脾、肺、肾中拯救出相应感染性病毒颗粒,并在脑、骨髓、心、胰、肠系膜中检到高拷贝BFV原病毒DNA存在.同时,血清学检测表明:感染兔在接受注射一个月后即产生高滴度抗病毒蛋白抗体,并维持该滴度水平直至实验终止,兔未表现任何可观病变.
장우포말병독(BFV3026)감염적세포경이연정맥주사토자,병이정상세포주사적토위대조.1년후처사,병독만구실험급PCR검측현시:토경일차주사즉가피BFV3026감염,병독엄범분포우감염토적다충장기중,통과공배양가종감염토혈、간、비、폐、신중증구출상응감염성병독과립,병재뇌、골수、심、이、장계막중검도고고패BFV원병독DNA존재.동시,혈청학검측표명:감염토재접수주사일개월후즉산생고적도항병독단백항체,병유지해적도수평직지실험종지,토미표현임하가관병변.
Rabbits were injected with a single intravenation of Bovine foamy virus 3026 (BFV3026)- infected cells in the ear and monitored for sera-conversion serologically up to 1 year. Results of the serological and virus-rescue assays indicated that all animals were persistently infected with BFV3026 and showed sustained BFV3026-specific humoral immune response. BFV3026 was rescued by co-cultivation with fetal bovine lung (FBL) cells from the spleen, kidney, lung, liver and peripheral blood leukocytes of the infected animals. Virus sequence in pol was recovered and amplified from the tissues where the virus was rescued from, such as from the heart, brain, bone marrow, mesentery and the pancreas. In addition, BFV transcripts could be detected in PBL by RT-PCR. This animal model might be useful for studying the interaction between BFV and BIV.