CAJ | 학술논문

Objective To investigate the role of cyclin-kinase inhibitor p27 on proliferation of mesangial cell(MC) induced by tumor necrosis factor α (TNF-α). Methods The p27 protein of MC lysate was detected with western blotting analysis. The degree of MC proliferation was estimated through [ 3 H] thymidine incorporation.The effect of reducing p27 expression on MC proliferation was analysed with p27 antisense oligodeoxynucleotide (ODN). Results TNF- α (200000U/L) decreased p27 level to ( 0. 6 + 0. 1 ) from ( 1.1 + 0. 1 ) of MC lysate cultured in serum free DMEM for 24h (P ＜0. 01 ) and increased [ 3H] thymidine incorporation to (2060 + 112 )from (685 + 53 ) cpm/well( P ＜0. 01 ). p27 antisense ODN transfection decreased p27 level of MC stimulated by TNF- α for 24h [ ( 0. 3 + 0. 1 ) vs ( 0. 6 + 0. 1 ) , P ＜ 0. 01 ] and increased [ 3 H] thymidine incorporation [ ( 2420 +130) vs (2060 + 112) cpm/well, P ＜0. 05]. Conclusion The decline of p27 protein maybe play an important role in MC proliferation induced by TNF-α.