CAJ | 학술논문

目的观察鞘内注射P物质(SP)拮抗剂spantide{[D-Arg1,D-Trp7,9,Leu11]-substance P}对炎性痛大鼠Ls节段脊髓后角一氧化氮合酶(NOS)表达和腰膨大一氧化氮(NO)含量的影响,以探讨痛及痛过敏时脊髓NOS表达和NO生成增多的机制.方法大鼠右后掌足底皮下注射5%甲醛0.2 mL诱发炎性痛及痛过敏,NADPH-d组化法观察脊髓后角NOS表达的变化,硝酸还原酶法测定NO含量的变化.结果皮下注射甲醛24h后,双侧L5节段脊髓后角NOS表达及腰膨大部位NO生成明显增加;注射甲醛前5 min鞘内注射spantide(5μg,10μL),则明显抑制甲醛所致的NOS表达及NO生成增加.结论初级传入末梢释放的SP在甲醛炎性痛及痛过敏时脊髓NOS表达及NO生成增多中发挥作用.
목적관찰초내주사P물질(SP)길항제spantide{[D-Arg1,D-Trp7,9,Leu11]-substance P}대염성통대서Ls절단척수후각일양화담합매(NOS)표체화요팽대일양화담(NO)함량적영향,이탐토통급통과민시척수NOS표체화NO생성증다적궤제.방법대서우후장족저피하주사5%갑철0.2 mL유발염성통급통과민,NADPH-d조화법관찰척수후각NOS표체적변화,초산환원매법측정NO함량적변화.결과피하주사갑철24h후,쌍측L5절단척수후각NOS표체급요팽대부위NO생성명현증가;주사갑철전5 min초내주사spantide(5μg,10μL),칙명현억제갑철소치적NOS표체급NO생성증가.결론초급전입말소석방적SP재갑철염성통급통과민시척수NOS표체급NO생성증다중발휘작용.
AIM To study the effect of spantide { [ D-Arg1,D-Trp7,9,Leu11]-substance P},an antagonist of substance P(SP),on the up-regulation of nitric oxide synthase(NOS)and nitric oxide(NO)in the spinal cord of rat during inflammatory pain and hyperalgesia.METHODS The inflammatory pain and hyperalgesia were induced by subcutaneous injection of 5 ％ fomalde hyde 0.2 mL into the right hind paw of the rats.NOS ex pression level was determined using NADPH-diaphorase histochemistry,and NO production was determined by ni trate reductase method.RESULTS The fomaldehyde injection significantly increased NOS expression level and NO production in the dorsal horn of the L5 segment of the spinal cord following pain and hyperalgesia.The increases reached its peak 24 h after the injection.Intrathecal injection of spantide(5 μg,10 μL)5 min prior to the fomaldehyde injection substantially inhibited the increases in NOS expression and NO production.CONCLUSION The release of SP resulted from nociceptive afferents plays an important role in the increases in NOS expression and NO production in the spinal cord of rat with pain and hyperalgesia induced by the fomaldehyde injection.