CAJ | 학술논문

目的进一步观察移植后内皮细胞(EC)抗切应力和抗钙化能力以及肌成纤维细胞迁移和自身修复能力.方法新鲜牛心包片经脱细胞、鞣制、改性后备用;A、B组试片上依序种植宿主肌成纤维细胞和EC,C组试片上不种植细胞,随后将3组试片分别移植于猪的腹主动脉壁上;2个月后对A组试片,12个月后对B、C组试片进行厚度、钙含量、扫描电镜及组织学的检测.结果 A、B组试片钙含量显著低于C组(P<0.05),但显著高于新鲜牛心包(P<0.05);A、B组试片钙含量差异无显著性(P>0.05);A、B组试片显著厚于C组试片(P<0.05).A、B组试片的厚度差异无显著性(P>0.05);B组试片80%～90%的表面覆盖EC,肌成纤维细胞已迁移至表层下支架的3/4;C组试片表面无细胞,肌成纤维细胞已迁移至表层下支架的1/4～1/3,大部分胶原纤维间隙消失.结论人工EC化使生物材料抗钙化能力增强;种植肌成纤维细胞使生物材料具有自身修复能力.但增厚的瓣叶是否影响其功能,尚有待进一步研究.
목적 진일보관찰이식후내피세포(EC)항절응력화항개화능력이급기성섬유세포천이화자신수복능력.방법 신선우심포편경탈세포、유제、개성후비용;A、B조시편상의서충식숙주기성섬유세포화EC,C조시편상불충식세포,수후장3조시편분별이식우저적복주동맥벽상;2개월후대A조시편,12개월후대B、C조시편진행후도、개함량、소묘전경급조직학적검측.결과 A、B조시편개함량현저저우C조(P<0.05),단현저고우신선우심포(P<0.05);A、B조시편개함량차이무현저성(P>0.05);A、B조시편현저후우C조시편(P<0.05).A、B조시편적후도차이무현저성(P>0.05);B조시편80%～90%적표면복개EC,기성섬유세포이천이지표층하지가적3/4;C조시편표면무세포,기성섬유세포이천이지표층하지가적1/4～1/3,대부분효원섬유간극소실.결론 인공EC화사생물재료항개화능력증강;충식기성섬유세포사생물재료구유자신수복능력.단증후적판협시부영향기공능,상유대진일보연구.
To further analyze the ability of resisting the shear-stress and anti-calcification of en-dothelial cells (EC),and further analyze the ability of migrating and self-repair of myofibroblasts.[Methods]The fresh bovine pericardia were acellularized,tanned and modified;Autologous myofibroblasts and Ecs were seeded on the patches of Group A and B sequentially;Group C was unseeded control group.Then the patches of all groups were implanted into the porcine abdominal aortic wall separately;The retrieved specimens of Group A were evaluat-ed by the studies of thickness,calcium content,scanning electron microscopy(SEM)and histological examination 2 months later,Group B and C were evaluated 12 months later.[Results]The specimen calcium of Group A and B was less than that of Group C significantly(P<0.05),but more than that of fresh pericardial group significantly(P<0.05);And the specimen calcium of Group A and B was not significantly different(P>0.05);The specimen thickness of Group A and B was thicker than that of Group C significantly(P<0.05),but it was not significantly different in Group A and B(P>0.05);In Group B,Ecs covered 80%～90%of the specimen surface,myofibroblasts proliferated and immigrated into 3/4 thickness of the matrix;In Group C,no cells were found on the surface of specimens,my-ofibroblasts proliferated and immigrated into 1/4～1/3 thickness of the matrix.and most of interfiber fissures disap-peared.[Conclusions] Artificial endothelialization of the biological material may improve the ability of preventing biopmsthetic calcification;The seeded myofibroblasts may offer the ability of self-repair of biopmstheses.But will the valve learlets with increased thickness interfere with the funotion?It's still to be determined.