CAJ | 학술논문

目的:建立痔消栓的HPLC含量测定方法.方法:采用HPLC法,以Kromasil C18(4.6 mm×250 mm,5μm)色谱柱为固定相,以乙腈-0.2%磷酸水溶液为流动相,梯度洗脱,流速1.0 mL·min-1,柱温23℃,检测波长为203 nm.结果:三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1分别在0.368～2.76μg,0.676～5.07 μg,0.812～6.09μg范围内线性关系良好,相关系数分别为0.999 9,0.999 9,0.999 8,平均加样回收率分别为98.53%,99.20%,99.35%,RSD分别为2.11%,1.88%,2.38%.结论:样品分离效果好,测定结果准确、可靠,重复性好,可用于痔消栓的质量控制.
목적:건립치소전적HPLC함량측정방법.방법:채용HPLC법,이Kromasil C18(4.6 mm×250 mm,5μm)색보주위고정상,이을정-0.2%린산수용액위류동상,제도세탈,류속1.0 mL·min-1,주온23℃,검측파장위203 nm.결과:삼칠조감R1、인삼조감Rg1、인삼조감Rb1분별재0.368～2.76μg,0.676～5.07 μg,0.812～6.09μg범위내선성관계량호,상관계수분별위0.999 9,0.999 9,0.999 8,평균가양회수솔분별위98.53%,99.20%,99.35%,RSD분별위2.11%,1.88%,2.38%.결론:양품분리효과호,측정결과준학、가고,중복성호,가용우치소전적질량공제.
Objective: To denvolop HPLC an method for the determination of the content of three saponins in Zhixiao suppositories. Methods: Analysis carriedout was performed on a Kromasil C18 column (4.6 mm×250 mm., 5μm)at 23℃. The mobile phases were methanol and 0.2% phosphoricacid solution in a gradient elution. The flow rate was set at 1.0 mL·min-1 and the ultraviolet detector was operated at 203 nm. Results: The linear ranges of notoginsenoside R1, ginsenoside Rg1 and ginsenoside Rb1 were 0.368～2.76μg,0.676～5.07 μg and 0.812～6.09μg,respectively. The average recoveries of the three saponins were 98.53%, 99.20% and 99.35%, respectively. The RSD (n=5) were 2.11%, 1.88% and 2.38%, respectively. Conclusion: The separation is a good and the results are accurate and reproducible. It can be used for the quality control of Zhixiao suppositories.