CAJ | 학술논문

以酸性纤维素酶产生菌绿色木霉(Trichoderma viride) WL 0512作为原始出发菌株,首先经自然分离筛选出一株产酶较稳定的菌株TVN-18,其羧甲基纤维素酶活(CMC酶活)达2765.8 U/g,滤纸酶活(FPA酶活)达48.5 U/g.再经真空微波和甲基磺酸乙酯(EMS)逐级诱变处理,获得了一株高产、稳产酸性纤维素酶的E6-1菌株,其CMC酶活达4396.6 U/g,FPA酶活达126.0 U/g,分别是菌株TVN-18的1.59倍和2.60倍.通过对固态发酵培养基麸皮和稻草比例、料水比以及初始pH值的优化,突变株的产酶能力进一步得到提高,其产的CMC酶活和FPA酶活分别提高了22.3%和22.4%.
이산성섬유소매산생균록색목매(Trichoderma viride) WL 0512작위원시출발균주,수선경자연분리사선출일주산매교은정적균주TVN-18,기최갑기섬유소매활(CMC매활)체2765.8 U/g,려지매활(FPA매활)체48.5 U/g.재경진공미파화갑기광산을지(EMS)축급유변처리,획득료일주고산、은산산성섬유소매적E6-1균주,기CMC매활체4396.6 U/g,FPA매활체126.0 U/g,분별시균주TVN-18적1.59배화2.60배.통과대고태발효배양기부피화도초비례、료수비이급초시pH치적우화,돌변주적산매능력진일보득도제고,기산적CMC매활화FPA매활분별제고료22.3%화22.4%.
A high yield cellulase producing strain Trichoderma viride TVN-18 was isolated by natural separation. After treated with vacuum microwave irradiation and ethyl methane-sulfonate, a higher yield acidic cellulase producing strain Trichoderma viride E6-1 was obtained. The CMCase was increased by 59% from 2765.8 U/g to 4396.6 U/g, and the FPase was increased by 160% from 48.5 U/g to 126.0 U/g. Its solid state fermentation (SSF) was performed by using different initial pH, ratios of rice straw to wheat bran and material to water. The CMCase and FPase of the strain E6-1 growing on the optimal SSF were increased by 22.3% and 22.4%, respectively.