中国实验动物学报
中國實驗動物學報
중국실험동물학보
ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA
2010年
1期
44-47
,共4页
李洪涛%肖东%顾为望%吴清洪%袁进%王万山%张嘉宁
李洪濤%肖東%顧為望%吳清洪%袁進%王萬山%張嘉寧
리홍도%초동%고위망%오청홍%원진%왕만산%장가저
西藏小型猪%细胞色素B基因%比较研究
西藏小型豬%細胞色素B基因%比較研究
서장소형저%세포색소B기인%비교연구
Tibet mini-pig%Cyt b gene%Comparative research
目的 对西藏小型猪Cyt b-基因序列进行分析,研究其遗传背景及其与国内家猪的亲缘关系.方法 提取西藏小型猪和巴马小型猪、贵州香猪、五指山猪的全基因组DNA,设计引物扩增Cyt b基因,测序后进行碱基序列比对,建立亲缘关系树,分析西藏小型猪的遗传背景.结果 西藏小型猪等国内部分品种猪的Cyt b基因序列与欧洲猪相比有14个变异位点;但是西藏小型猪与国内品种猪相比存在两个特殊碱基位点即在420位点T-c转换的同时在883位点存在G-A转换.结论 西藏小型猪与巴马小型猪、贵州香猪、五指山猪等国内家猪有很近的亲缘关系.同时进一步证实西藏小型猪群体内存在一定的遗传分化.
目的 對西藏小型豬Cyt b-基因序列進行分析,研究其遺傳揹景及其與國內傢豬的親緣關繫.方法 提取西藏小型豬和巴馬小型豬、貴州香豬、五指山豬的全基因組DNA,設計引物擴增Cyt b基因,測序後進行堿基序列比對,建立親緣關繫樹,分析西藏小型豬的遺傳揹景.結果 西藏小型豬等國內部分品種豬的Cyt b基因序列與歐洲豬相比有14箇變異位點;但是西藏小型豬與國內品種豬相比存在兩箇特殊堿基位點即在420位點T-c轉換的同時在883位點存在G-A轉換.結論 西藏小型豬與巴馬小型豬、貴州香豬、五指山豬等國內傢豬有很近的親緣關繫.同時進一步證實西藏小型豬群體內存在一定的遺傳分化.
목적 대서장소형저Cyt b-기인서렬진행분석,연구기유전배경급기여국내가저적친연관계.방법 제취서장소형저화파마소형저、귀주향저、오지산저적전기인조DNA,설계인물확증Cyt b기인,측서후진행감기서렬비대,건립친연관계수,분석서장소형저적유전배경.결과 서장소형저등국내부분품충저적Cyt b기인서렬여구주저상비유14개변이위점;단시서장소형저여국내품충저상비존재량개특수감기위점즉재420위점T-c전환적동시재883위점존재G-A전환.결론 서장소형저여파마소형저、귀주향저、오지산저등국내가저유흔근적친연관계.동시진일보증실서장소형저군체내존재일정적유전분화.
Objective To analyze the Cyt b gene sequences in Tibet mini-pigs and clarify the differences and genetic relationship with other Chinese pigs.Method The sequence of Cyt b gene was amplified from genome DNA of Tibet mini-pig,Bama miniature pigs,Guizhou xiang pigs and Wuzhishan (WZS) pigs.After sequencing,the base sequences were compared and analysed.The blood relationship tree and evolution position of Tibet mini-pig were established.Result There were 14 mutation sites between domestic pigs in China and pigs from Europe.Besides there was a significant differenee in two nucleotide site:a T→C switch in site 420 and the G→A switch in site 883 at the same time.Conclusion Chinese pigs include Bama miniature pigs,Guizhou xiang pigs and WZS pigs,have a very close blood relationship with some of Tibet mini-pigs.It has been confirmed that there is a certain genetic differentiation in the Tibet mini-pig.
