国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2011年
10期
1229-1232
,共4页
乙型肝炎病毒%血清标志物%HBV-DNA%相关性
乙型肝炎病毒%血清標誌物%HBV-DNA%相關性
을형간염병독%혈청표지물%HBV-DNA%상관성
HBV-M%HBV-DNA%Correlation
目的 探讨不同的乙肝血清标志物(HBV-M)模式与HBV-DNA定量检测结果之间的关系.方法 选择800例经酶联免疫吸附试验(ELISA)检测HBsAg结果为阳性的血清样本,采用实时荧光定量PCR技术(FQ-PCR)检测其HBV-DNA含量;并根据患者HBV-M的不同模式进行分组统计,分析探讨HBV-M模式与HBV-DNA含量之间的关系.结果 不同HBV-M模式中HBV-DNA阳性检出率和含量存在明显差异.①"大三阳"组HBV-DNA含量以中、高拷贝为主;②"小三阳"组及HBsAg(+)&Anti-HBc(+)组HBV-DNA含量以中、低拷贝为主;③单纯HBsAg(+)组HBV-DNA的检出率仅为2.98%.④HBeAg(+)血样中HBV-DNA的阳性检出率高达98.33%(118/120),两者之间存在明显正相关(r=0.993).结论 HBV-M与HBV-DNA的检测都能反映HBV感染、传染性及体内复制的情况;HBeAg与HBV-DNA含量存在明显正相关.两者联合检测对乙肝的临床诊疗具有重要指导意义.
目的 探討不同的乙肝血清標誌物(HBV-M)模式與HBV-DNA定量檢測結果之間的關繫.方法 選擇800例經酶聯免疫吸附試驗(ELISA)檢測HBsAg結果為暘性的血清樣本,採用實時熒光定量PCR技術(FQ-PCR)檢測其HBV-DNA含量;併根據患者HBV-M的不同模式進行分組統計,分析探討HBV-M模式與HBV-DNA含量之間的關繫.結果 不同HBV-M模式中HBV-DNA暘性檢齣率和含量存在明顯差異.①"大三暘"組HBV-DNA含量以中、高拷貝為主;②"小三暘"組及HBsAg(+)&Anti-HBc(+)組HBV-DNA含量以中、低拷貝為主;③單純HBsAg(+)組HBV-DNA的檢齣率僅為2.98%.④HBeAg(+)血樣中HBV-DNA的暘性檢齣率高達98.33%(118/120),兩者之間存在明顯正相關(r=0.993).結論 HBV-M與HBV-DNA的檢測都能反映HBV感染、傳染性及體內複製的情況;HBeAg與HBV-DNA含量存在明顯正相關.兩者聯閤檢測對乙肝的臨床診療具有重要指導意義.
목적 탐토불동적을간혈청표지물(HBV-M)모식여HBV-DNA정량검측결과지간적관계.방법 선택800례경매련면역흡부시험(ELISA)검측HBsAg결과위양성적혈청양본,채용실시형광정량PCR기술(FQ-PCR)검측기HBV-DNA함량;병근거환자HBV-M적불동모식진행분조통계,분석탐토HBV-M모식여HBV-DNA함량지간적관계.결과 불동HBV-M모식중HBV-DNA양성검출솔화함량존재명현차이.①"대삼양"조HBV-DNA함량이중、고고패위주;②"소삼양"조급HBsAg(+)&Anti-HBc(+)조HBV-DNA함량이중、저고패위주;③단순HBsAg(+)조HBV-DNA적검출솔부위2.98%.④HBeAg(+)혈양중HBV-DNA적양성검출솔고체98.33%(118/120),량자지간존재명현정상관(r=0.993).결론 HBV-M여HBV-DNA적검측도능반영HBV감염、전염성급체내복제적정황;HBeAg여HBV-DNA함량존재명현정상관.량자연합검측대을간적림상진료구유중요지도의의.
Objective To analyze the correlation between the lovel of HBV semiogloat markers and HBV-DNA.Mothods Serum samples of 800 patients from Xinyi Pepole's Hospital clinic department suffering HBV were detected by ELISA and HBV-DNA were detected by FQ-PCR.then analyze the correlation between HBV serological markers and HBV-DNA.Results The HBV-DNA was detoeted in some HBV serological markerg groups and each group was different in the result.HBsAg positive.such as HBsAg,HBeAg and Anti-HBc positive samples has higher amount of copies of HBV-DNA;the low level of HBV-DNA copies were detected in the group of HBsAg.HBeAg or Anti-HBe,Anti-HBo lmsitive; and the HBV-DNA copies rate is 2.98%in the single HBsAg positive.Among 120 HBe Ag positive samples.there were 118 HBV-DNA positive,the positive rate of HBV-DNA was 98.33%and the level of HBeAg positive wail correlated to HBV-DNA(r=0.993).Conclusion Both the detection of HBeAg and HBV-DNA cab reflect the active level of duplicate of HBV-M.The HBV-DNA level showed all obvious correlation to the HBeAg contend in Bera.
