水产科学
水產科學
수산과학
FISHERIES SCIENCE
2010年
3期
131-136
,共6页
C、N、P比例%盐藻%生长%β-胡萝卜素
C、N、P比例%鹽藻%生長%β-鬍蘿蔔素
C、N、P비례%염조%생장%β-호라복소
ratio among C,N and P%Dunaliella salina%growth%β-carotene
采用正交设计法研究了C、N、P比例对2株盐藻OUN04和 OUN05生长、β-胡萝卜素积累、叶绿素a合成、脂肪酸组成、蛋白质含量的影响.试验结果表明,适当调节C、N、P比例可以促进盐藻OUN04生长、β-胡萝卜素积累、叶绿素a合成,盐藻 OUN04最大细胞密度114.2×10~4个/ml、β-胡萝卜素最大含量100.4 mg/g、叶绿素a最高含量144.6 mg/g分别出现在C、N、P比例为12∶1∶0.05、12∶2∶0.1、12∶1∶0.05的处理组中,且均与对照组差异显著; 适当调节C、N、P比例也可以提高盐藻OUN04脂肪酸和蛋白质含量.盐藻OUN05细胞最大密度101.2×10~4 个/ml、β-胡萝卜素最高含量109.2 mg/g、叶绿素a最高含量108.7 mg/g分别出现在C、N、P比例为6∶2∶0.05、6∶0.5∶0.1、12∶2∶0.1的处理组中,且均与对照组差异显著;最大蛋白含量34.41%出现在6∶1∶0.05的处理组中,但与对照组(33.17%)差异不显著.
採用正交設計法研究瞭C、N、P比例對2株鹽藻OUN04和 OUN05生長、β-鬍蘿蔔素積纍、葉綠素a閤成、脂肪痠組成、蛋白質含量的影響.試驗結果錶明,適噹調節C、N、P比例可以促進鹽藻OUN04生長、β-鬍蘿蔔素積纍、葉綠素a閤成,鹽藻 OUN04最大細胞密度114.2×10~4箇/ml、β-鬍蘿蔔素最大含量100.4 mg/g、葉綠素a最高含量144.6 mg/g分彆齣現在C、N、P比例為12∶1∶0.05、12∶2∶0.1、12∶1∶0.05的處理組中,且均與對照組差異顯著; 適噹調節C、N、P比例也可以提高鹽藻OUN04脂肪痠和蛋白質含量.鹽藻OUN05細胞最大密度101.2×10~4 箇/ml、β-鬍蘿蔔素最高含量109.2 mg/g、葉綠素a最高含量108.7 mg/g分彆齣現在C、N、P比例為6∶2∶0.05、6∶0.5∶0.1、12∶2∶0.1的處理組中,且均與對照組差異顯著;最大蛋白含量34.41%齣現在6∶1∶0.05的處理組中,但與對照組(33.17%)差異不顯著.
채용정교설계법연구료C、N、P비례대2주염조OUN04화 OUN05생장、β-호라복소적루、협록소a합성、지방산조성、단백질함량적영향.시험결과표명,괄당조절C、N、P비례가이촉진염조OUN04생장、β-호라복소적루、협록소a합성,염조 OUN04최대세포밀도114.2×10~4개/ml、β-호라복소최대함량100.4 mg/g、협록소a최고함량144.6 mg/g분별출현재C、N、P비례위12∶1∶0.05、12∶2∶0.1、12∶1∶0.05적처리조중,차균여대조조차이현저; 괄당조절C、N、P비례야가이제고염조OUN04지방산화단백질함량.염조OUN05세포최대밀도101.2×10~4 개/ml、β-호라복소최고함량109.2 mg/g、협록소a최고함량108.7 mg/g분별출현재C、N、P비례위6∶2∶0.05、6∶0.5∶0.1、12∶2∶0.1적처리조중,차균여대조조차이현저;최대단백함량34.41%출현재6∶1∶0.05적처리조중,단여대조조(33.17%)차이불현저.
The effects of ratio among C, N, and P on the growth and biochemical compositions in two strains of alga Dunaliella salina called OUN04, and OUN05 were investigated in an orthogonal design test. The maximum cell density of 114.2×10~4 cell/ml, β-carotene content of 100.4 mg/g and chlorophyll-a content of 144.6 mg/g were detected in the alga OUN04 exposed to the media with the ratios of C∶N∶P=12/1/0.05, 12/2/0.1, and 12/1/0.05, respectively, significantly different from those in the control groups. The maximum content of total fatty acids was found to be 86.20% in the OUN04, significantly higher than that in the control group(81.02%). The alga OUN04 had the maximum protein content of 32.44% when the alga was exposed to the medium with the ratios of C∶N∶P=6/1/0.05, insignificantly different from that in the control group. For the alga OUN05, the maximum cell density of 101.2×10~4 cell/ml, β-carotene content of 109.2 mg/g and chlorophyll-a content of 108.7 mg/g were detected in the treated groups with C∶N∶P=6/2/0.05, 6/0.5/0.1, and 12/2/0.1, respectively, significantly different from those in the control groups. The maximum protein content of 34.41% was observed in the C∶N∶P=6/1/0.05 group of, insignificantly different from that in the control group(33.17%).
