中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2009年
9期
689-692
,共4页
肖勇%余保平%胡柳丹%余志
肖勇%餘保平%鬍柳丹%餘誌
초용%여보평%호류단%여지
胆结石%胆固醇%肌条
膽結石%膽固醇%肌條
담결석%담고순%기조
Cholelithiasis%Cholesterol%Muscle strip
目的 探讨胆固醇与胆囊动力的关系及其在胆固醇结石形成中的作用.方法 构造豚鼠胆固醇结石模型,检测各组豚鼠血清胆固醇(TC)及胆汁胆固醇(BC)含量;利用灌流法加药,张力换能器记录胆囊收缩素对各组离体胆囊肌条张力的变化,并初步探讨胆固醇对胆囊肌条的作用机制.结果 A组(正常豚鼠)无结石发生,B、C两组(致石饲料4周、8周)共有13例结石发生;B、C两组TC和BC升高(与A组相比P<0.05),加入CCK后收缩振幅均增加,呈浓度依赖性.且与TC、BC相关(P<0.05);同A组相比,B、C及D(胆固醇孵育后胆囊肌条)三组对CCK效应值变小,起作用时间慢(P<0.05);CCK对胆囊离体肌条的作用可以部分被胆固醇抑制(变化为69.2%,P<0.05),而胆固醇的影响可以部分被阿托品、尼莫地平抑制(变化分别为64.2%、62.1%,P<0.05),不被TTX影响(P>0.05).结论 TC及BC增高的同时胆囊肌条肌张力降低及对CCK的敏感性降低,成石率增高;胆固醇孵育后肌条肌张力降低及对CCK的敏感性降低;胆固醇可能与胆囊动力及胆固醇结石形成相关.
目的 探討膽固醇與膽囊動力的關繫及其在膽固醇結石形成中的作用.方法 構造豚鼠膽固醇結石模型,檢測各組豚鼠血清膽固醇(TC)及膽汁膽固醇(BC)含量;利用灌流法加藥,張力換能器記錄膽囊收縮素對各組離體膽囊肌條張力的變化,併初步探討膽固醇對膽囊肌條的作用機製.結果 A組(正常豚鼠)無結石髮生,B、C兩組(緻石飼料4週、8週)共有13例結石髮生;B、C兩組TC和BC升高(與A組相比P<0.05),加入CCK後收縮振幅均增加,呈濃度依賴性.且與TC、BC相關(P<0.05);同A組相比,B、C及D(膽固醇孵育後膽囊肌條)三組對CCK效應值變小,起作用時間慢(P<0.05);CCK對膽囊離體肌條的作用可以部分被膽固醇抑製(變化為69.2%,P<0.05),而膽固醇的影響可以部分被阿託品、尼莫地平抑製(變化分彆為64.2%、62.1%,P<0.05),不被TTX影響(P>0.05).結論 TC及BC增高的同時膽囊肌條肌張力降低及對CCK的敏感性降低,成石率增高;膽固醇孵育後肌條肌張力降低及對CCK的敏感性降低;膽固醇可能與膽囊動力及膽固醇結石形成相關.
목적 탐토담고순여담낭동력적관계급기재담고순결석형성중적작용.방법 구조돈서담고순결석모형,검측각조돈서혈청담고순(TC)급담즙담고순(BC)함량;이용관류법가약,장력환능기기록담낭수축소대각조리체담낭기조장력적변화,병초보탐토담고순대담낭기조적작용궤제.결과 A조(정상돈서)무결석발생,B、C량조(치석사료4주、8주)공유13례결석발생;B、C량조TC화BC승고(여A조상비P<0.05),가입CCK후수축진폭균증가,정농도의뢰성.차여TC、BC상관(P<0.05);동A조상비,B、C급D(담고순부육후담낭기조)삼조대CCK효응치변소,기작용시간만(P<0.05);CCK대담낭리체기조적작용가이부분피담고순억제(변화위69.2%,P<0.05),이담고순적영향가이부분피아탁품、니막지평억제(변화분별위64.2%、62.1%,P<0.05),불피TTX영향(P>0.05).결론 TC급BC증고적동시담낭기조기장력강저급대CCK적민감성강저,성석솔증고;담고순부육후기조기장력강저급대CCK적민감성강저;담고순가능여담낭동력급담고순결석형성상관.
Objective To investigate the relationship between cholesterol, the dynamic of gallbladder and their role in cholesterol gallbladder formation by establishing an animal model of cholesterol gallstone. Methods The model of cholesterol gallstone was prepared by feeding guinea pig with stone-leading forage. The contents of serum total cholesterol (TC) and bile cholesterol (BC) of subgroup A [the normal guinea pigs], subgroup B [stone-leading forage for four weeks], subgroup C [stone-leading forage for eight weeks] were detected respectively. The effect of cholecystokinin-octopeptide (CCK-8) on constrictions of extracor-poreal gallbladder muscle strip was observed in subgroup A, B, C and D [gallbladder strip incubation in cho-lesterol 2 hours] to probe its possible mechanism. Results No gallstone was found in subgroup A. A total of 13 cholesterol gallstone guinea pigs was seen in subgroup B and group C. With the increase of TC and BC, the amplitude of contractions was upregulated by CCK-8 in a dose-independent manner in subgroup A, B, C and D (P<0.05). As compared with subgroup A, there was significant change in subgroup B, subgroup C and subgroup D (P<0. 05). The response of CCK-8 on the constrictions of extracorporeal gallbladder mus-cle strip could be inhibited partly by cholesterol (change 69.2%, P<0. 05). The effects of cholesterol on the constrictions of extracorporeal gallbladder muscle strip could be inhibited partially by atropine and nimo-dipine (changed for 64. 2%, 62. 1% respectively, P<0. 05). However, it could not be affected by TTX (P<0. 05). Conclusion With increase in the contents of TC and BC, gallbladder muscle strip tension and it's sensitivity to CCK-8 are decreased and the ratio of gallstone formation is increased. Cholesterol can pro-mote the formation of cholesterol stone by resulting in a defective gallbladder muscle strip contraction.
