中华超声影像学杂志
中華超聲影像學雜誌
중화초성영상학잡지
CHINESE JOURNAL OF ULTRASONOGRAPHY
2010年
8期
711-714
,共4页
杨嘉嘉%陈志奎%林礼务%薛恩生%何以敉%俞丽云
楊嘉嘉%陳誌奎%林禮務%薛恩生%何以敉%俞麗雲
양가가%진지규%림례무%설은생%하이미%유려운
超声检查%乳腺肿瘤%紫杉酚%温敏型凝胶
超聲檢查%乳腺腫瘤%紫杉酚%溫敏型凝膠
초성검사%유선종류%자삼분%온민형응효
Ultrasonography%Breast neoplasms%Paclitaxel%Thermosensitive hydrogel
目的 探讨超声介入紫杉醇温敏型凝胶缓释治疗大鼠乳腺癌的疗效及相关作用机制.方法 制备紫杉醇温敏型凝胶;建立大鼠皮下移植乳腺癌模型,随机选取合格模型40只,随机分成4组,在超声引导下分别于瘤体内注射生理盐水、温敏型凝胶、紫杉醇注射液、紫杉醇温敏型凝胶,观察肿瘤的超声影像学改变,第14 d处死大鼠剥离肿瘤,计算抑瘤率,进行病理学检查及RT-PCR法检测肿瘤组织bcl-2、bax基因表达的变化.结果 紫杉醇凝胶组治疗后其平均瘤质量[(16.04±2.82)g]较紫杉醇对照组明显下降[27.45±5.13)g,P＜0.05],抑瘤率达62.77%,超声显示见血流信号明显减少,病理显示瘤细胞广泛坏死,此外,凋亡相关基因bcl-2表达明显下调,而bax表达明显上调.结论 超声介入肿瘤内注射紫杉醇温敏型凝胶缓释治疗大鼠乳腺癌疗效显著,其机制可能与抑制肿瘤增殖及诱导细胞凋亡有关.
目的 探討超聲介入紫杉醇溫敏型凝膠緩釋治療大鼠乳腺癌的療效及相關作用機製.方法 製備紫杉醇溫敏型凝膠;建立大鼠皮下移植乳腺癌模型,隨機選取閤格模型40隻,隨機分成4組,在超聲引導下分彆于瘤體內註射生理鹽水、溫敏型凝膠、紫杉醇註射液、紫杉醇溫敏型凝膠,觀察腫瘤的超聲影像學改變,第14 d處死大鼠剝離腫瘤,計算抑瘤率,進行病理學檢查及RT-PCR法檢測腫瘤組織bcl-2、bax基因錶達的變化.結果 紫杉醇凝膠組治療後其平均瘤質量[(16.04±2.82)g]較紫杉醇對照組明顯下降[27.45±5.13)g,P＜0.05],抑瘤率達62.77%,超聲顯示見血流信號明顯減少,病理顯示瘤細胞廣汎壞死,此外,凋亡相關基因bcl-2錶達明顯下調,而bax錶達明顯上調.結論 超聲介入腫瘤內註射紫杉醇溫敏型凝膠緩釋治療大鼠乳腺癌療效顯著,其機製可能與抑製腫瘤增殖及誘導細胞凋亡有關.
목적 탐토초성개입자삼순온민형응효완석치료대서유선암적료효급상관작용궤제.방법 제비자삼순온민형응효;건립대서피하이식유선암모형,수궤선취합격모형40지,수궤분성4조,재초성인도하분별우류체내주사생리염수、온민형응효、자삼순주사액、자삼순온민형응효,관찰종류적초성영상학개변,제14 d처사대서박리종류,계산억류솔,진행병이학검사급RT-PCR법검측종류조직bcl-2、bax기인표체적변화.결과 자삼순응효조치료후기평균류질량[(16.04±2.82)g]교자삼순대조조명현하강[27.45±5.13)g,P＜0.05],억류솔체62.77%,초성현시견혈류신호명현감소,병리현시류세포엄범배사,차외,조망상관기인bcl-2표체명현하조,이bax표체명현상조.결론 초성개입종류내주사자삼순온민형응효완석치료대서유선암료효현저,기궤제가능여억제종류증식급유도세포조망유관.
Objective To prepare a sustained-delivery of paclitaxel-loaded thermosensitive hydrogel for the treatment of breast cancer by ultrasound-guided intratumoral injection,and probe the antitumor effect and mechanism of the new method. Methods Paclitaxel-loaded thermosensitive hydrogel was prepared. A total of 40 rats bearing subcutaneous breast tumor were randomized into 4 groups. Each group of rats were administered ultrasound-guided intratumoral injection of different agents as follows: salin, thermosensitive hydrogel, paclitaxel injection and paclitaxel-loaded thermosensitive hydrogel. The tumor nodules were scanned by high frequence ultrasound. The rats were sacrificed on the 14th day,and then the tumors were stripped to calculate the growth inhibitory rate. Histological examinations were undergone and RT-PCR was performed for the detection of bcl-2 and bax expression. Results Greater decrease in average tumor weight was found in paclitaxel-gel group [(16. 04 ± 2. 82)g], compared with that in paclitaxel group [(27.45 ±5.13)g, P ＜0. 05],and tumor growth inhibitory rate in the group was 62.77 %. In this group, blood flow signals reduction was detected on ultrasound and extensive necrosis was found in histological examination. In addition,down-regulation in bcl-2 mRNA expression and up-regulation in bax were found. Conclusions Ultrasound-guided percutaneous intratumoral injection of paclitaxel-loaded thermosensitive hydrogel may provide an effective method for breast tumor ablation therapy. The mechanism may be related to the inhibition of proliferation and induction of apoptosis.
