南方医科大学学报
南方醫科大學學報
남방의과대학학보
JOURNAL OF SOUTHERN MEDICAL UNIVERSITY
2011年
10期
1641-1648
,共8页
艾瑞婷%吴少瑜%文晓芸%徐伟%吕琳%饶进军%吴曙光
艾瑞婷%吳少瑜%文曉蕓%徐偉%呂琳%饒進軍%吳曙光
애서정%오소유%문효예%서위%려림%요진군%오서광
miRNA%人肝癌HepG2细胞%细胞增殖%多酚化合物%BJA32515
miRNA%人肝癌HepG2細胞%細胞增殖%多酚化閤物%BJA32515
miRNA%인간암HepG2세포%세포증식%다분화합물%BJA32515
microRNA%human HepG2 hepatocarcinoma cells%cell proliferation%ellagitannin
目的 miRNA在细胞的增殖,分化和凋亡中起重要作用.1,3,4-三-O-没食子酰基-6-O-咖啡酰基-β-D-吡喃葡萄糖(BJA32515)是从日本蛇菰中分离的天然多酚化合物,该化合物对肿瘤细胞的增殖和miRNA的影响未见报道.本文旨在研究BJA32515对人肝癌细胞HepG2增殖的抑制作用以及对miRNA表达的影响.方法 采用CCK-8的方法检测HepG2细胞的增殖；用流式细胞法检测HepG2细胞的凋亡；采用miRNA芯片分析方法测定HepG2细胞的miRNA表达以及用RT-PCR的方法验证miRNA的表达.结果 BJA32515以时间和剂量依赖的方式抑制HepG2细胞的增殖,并促进细胞的凋亡.miRNA芯片结果显示,BJA32515能诱导HepG2细胞33个miRNA的表达上调以及59个miRNA的下调.RT-PCR结果也证实BJA32515 可诱导let-7a和miR-29a的上调以及miR-373和miR-197的下调,与芯片分析的结果一致.结论 BJA32515抑制HepG2细胞增殖的作用机制与miRNA的表达调控有关.
目的 miRNA在細胞的增殖,分化和凋亡中起重要作用.1,3,4-三-O-沒食子酰基-6-O-咖啡酰基-β-D-吡喃葡萄糖(BJA32515)是從日本蛇菰中分離的天然多酚化閤物,該化閤物對腫瘤細胞的增殖和miRNA的影響未見報道.本文旨在研究BJA32515對人肝癌細胞HepG2增殖的抑製作用以及對miRNA錶達的影響.方法 採用CCK-8的方法檢測HepG2細胞的增殖；用流式細胞法檢測HepG2細胞的凋亡；採用miRNA芯片分析方法測定HepG2細胞的miRNA錶達以及用RT-PCR的方法驗證miRNA的錶達.結果 BJA32515以時間和劑量依賴的方式抑製HepG2細胞的增殖,併促進細胞的凋亡.miRNA芯片結果顯示,BJA32515能誘導HepG2細胞33箇miRNA的錶達上調以及59箇miRNA的下調.RT-PCR結果也證實BJA32515 可誘導let-7a和miR-29a的上調以及miR-373和miR-197的下調,與芯片分析的結果一緻.結論 BJA32515抑製HepG2細胞增殖的作用機製與miRNA的錶達調控有關.
목적 miRNA재세포적증식,분화화조망중기중요작용.1,3,4-삼-O-몰식자선기-6-O-가배선기-β-D-필남포도당(BJA32515)시종일본사고중분리적천연다분화합물,해화합물대종류세포적증식화miRNA적영향미견보도.본문지재연구BJA32515대인간암세포HepG2증식적억제작용이급대miRNA표체적영향.방법 채용CCK-8적방법검측HepG2세포적증식；용류식세포법검측HepG2세포적조망；채용miRNA심편분석방법측정HepG2세포적miRNA표체이급용RT-PCR적방법험증miRNA적표체.결과 BJA32515이시간화제량의뢰적방식억제HepG2세포적증식,병촉진세포적조망.miRNA심편결과현시,BJA32515능유도HepG2세포33개miRNA적표체상조이급59개miRNA적하조.RT-PCR결과야증실BJA32515 가유도let-7a화miR-29a적상조이급miR-373화miR-197적하조,여심편분석적결과일치.결론 BJA32515억제HepG2세포증식적작용궤제여miRNA적표체조공유관.
Background MicroRNAs (miRNAs) play important roles in cell proliferation,differentiation and apoptosis.1,3,4-tri-O-galloyl-6-O-caffeoyl-β-D-glucopyranose (BJA32515) is a new natural ellagitannin compound extracted from Balanophora Japonica MAKINO.The effect of BJA32515 on the expression of miRNAs in cancer cells has not yet been explored.Objective The present study was carried out to examine the changes in miRNA expression profiles in human HepG2 hepatocarcinoma cells following BJA32515 exposure.Methods The proliferation of BJA32515-exposed HepG2 cells was assessed using a colorimetric assay (cell counting kit-8).The miRNA expression profile of the cancer cells was analyzed using a miRNA array and quantitative real-time PCR.Apoptosis was assessed by annexin V and propidium iodide staining.Results BJA32515 inhibited the cell proliferation and increased apoptosis in HepG2 cancer cells.The exposure to BJA32515 also caused alterations in the miRNA expression profile in the cells,with 33 miRNAs upregulated and 59 down-regulated.The up-regulation of let-7a and miR-29a and the down-regulation of miR-373 and miR-197 were verified by quantitative real-time PCR.Conclusion BJA32515-modifed miRNA expression may mediate the antiproliferative effect of this compound in HepC2 cancer cells.
