CAJ | 학술논문

[目的]构建干旱和盐碱共胁迫的玉米cDNA文库,对细胞功能相关基因表达进行初步分析。[方法]提取处理玉米YQ7-96品系的雌雄分化期（12片叶龄）的混合组织（叶、茎和花芽）总RNA,运用SMART技术构建pDNR-LIB载体的cDNA文库,并随机挑选克隆进行EST序列的BlastX比对和MIPS归类分析。[结果]随机挑取3 027个cDNA克隆进行测序,94.45%的EST长度大于400 bp,共获得1 861个单基因簇,其中维持正常生理活动的基因占65.36%;参与细胞内运输、细胞内信号传导、细胞防御和细胞循环和DNA代谢过程的基因分别占9.26%、6.58%、2.63%和3.16%。[结论]在成功构建干旱和盐碱共胁迫的玉米cDNA文库的基础上,对产生的EST进行了测序和分析归类,筛选出了细胞功能相关EST,为后续研究奠定了基础。
[목적]구건간한화염감공협박적옥미cDNA문고,대세포공능상관기인표체진행초보분석。[방법]제취처리옥미YQ7-96품계적자웅분화기（12편협령）적혼합조직（협、경화화아）총RNA,운용SMART기술구건pDNR-LIB재체적cDNA문고,병수궤도선극륭진행EST서렬적BlastX비대화MIPS귀류분석。[결과]수궤도취3 027개cDNA극륭진행측서,94.45%적EST장도대우400 bp,공획득1 861개단기인족,기중유지정상생리활동적기인점65.36%;삼여세포내운수、세포내신호전도、세포방어화세포순배화DNA대사과정적기인분별점9.26%、6.58%、2.63%화3.16%。[결론]재성공구건간한화염감공협박적옥미cDNA문고적기출상,대산생적EST진행료측서화분석귀류,사선출료세포공능상관EST,위후속연구전정료기출。
[Objective] The research aimed to construct maize cDNA library under the co-stress of drought,salt and alkali,and initially analyze the expression of gene which related to the cellular function.[Method] The total RNA of mixed tissue（leaf,stem and blossom bud） of maize YQ7-96 in the male and female differentiation period（12-leaf age） was extracted.By using SMART technology,cDNA library of pDNR-LIB vector was constructed.BlastX contrast and MIPS classification analysis of EST sequence were carried out by randomly picking the clone.[Result] 3 027 cDNA clones were picked out to sequence.The length of 94.45% EST was bigger than 400 bp,and 1 861 single gene clusters were obtained.The gene which maintained the normal physiological activity occupied 65.36%.The genes which involved in the intracellular transportation,signal transduction,cell defense and cycle,DNA metabolic process were respectively 9.26%,6.58%,2.63% and 3.16%.[Conclusion] Based on the successful construction of maize cDNA library under the co-stress of drought,salt and alkali,EST was sequenced,analyzed and classified.EST which related to the cellular function was screened.It laid the foundation for the following research.