CAJ | 학술논문

目的探讨VEGF、bFGF、TGFβ1在非小细胞肺癌的表达及与其生物学行为特征的关系.方法应用链霉菌抗生素蛋白-过氧化物酶连结(streptavidin-perosidase,SP)免疫组化方法检测VEGF、bFGF、TGFβ1及CD34在50例非小细胞肺癌的表达,并进行微血管密度计数,以18例癌旁组织为对照.结果 VEGF、bFGF、TGFβ1的阳性表达率分别为68%、58%、60%,VEGF、bFGF、TGFβ1表达均与NSCLC的临床分期(χ2值分别为4.535、3.864、3.852,P＜0.05)、淋巴结转移有关(χ2值分别为4.235、3.876、4.151,P＜0.05),而TGFβ1,表达还与组织学类型有关(χ2=4.443,P＜0.05),鳞癌比腺癌表达率高.MVD表达与NSCLC的临床分期、淋巴结转移有关(t值分别为2.312和2.865,P＜0.05),与组织学类型、组织分化程度无关;且四者表达在癌组织与癌旁组织之间均有显著性统计学意义.VEGF、bFGF表达与MVD有关(t值分别为2.518和3.679,P＜0.05);TGFB.表达与MVD无显著关系.结论 VEGF、bFGF、TGFβ1在NSCLC均有很好的表达,并且其表达与NSCLC的生长、进展、转移等生物学关系密切;VEGF、bFGF在肿瘤血管形成、转移中起重要作用,TGFβ1可能与机体免疫功能降低及肿瘤细胞免疫逃逸有关.
목적 탐토VEGF、bFGF、TGFβ1재비소세포폐암적표체급여기생물학행위특정적관계.방법 응용련매균항생소단백-과양화물매련결(streptavidin-perosidase,SP)면역조화방법검측VEGF、bFGF、TGFβ1급CD34재50례비소세포폐암적표체,병진행미혈관밀도계수,이18례암방조직위대조.결과 VEGF、bFGF、TGFβ1적양성표체솔분별위68%、58%、60%,VEGF、bFGF、TGFβ1표체균여NSCLC적림상분기(χ2치분별위4.535、3.864、3.852,P＜0.05)、림파결전이유관(χ2치분별위4.235、3.876、4.151,P＜0.05),이TGFβ1,표체환여조직학류형유관(χ2=4.443,P＜0.05),린암비선암표체솔고.MVD표체여NSCLC적림상분기、림파결전이유관(t치분별위2.312화2.865,P＜0.05),여조직학류형、조직분화정도무관;차사자표체재암조직여암방조직지간균유현저성통계학의의.VEGF、bFGF표체여MVD유관(t치분별위2.518화3.679,P＜0.05);TGFB.표체여MVD무현저관계.결론 VEGF、bFGF、TGFβ1재NSCLC균유흔호적표체,병차기표체여NSCLC적생장、진전、전이등생물학관계밀절;VEGF、bFGF재종류혈관형성、전이중기중요작용,TGFβ1가능여궤체면역공능강저급종류세포면역도일유관.
Objective To evaluate the expression of vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF)and transforming growth factor betal(TGFβ1)in non-small cell lung cancer (NSCLC)and to investigate its relationship to histopathological features.Methods The expression of VEGF,bFGF,TGFβ1 and CD34 were examined in 50 specimens of NSCLC and 18 specimens of para-cancerous tissue by using SP immunohistochemistry staining.Assessment of microvessel density(MVD)was performed.Results The positive rate of VEGF,bFGF,TGFβ1 was 68%.The expression of VEGF was related to clinical classification and lymph nodes metastasis.The difference was significant among the groups(χ2=4.535 and 4.235,P＜0.05).The expression of VEGF had no significant relationship with some pathological characteristics such as histological types and pathological grade.The positive rate of bFGF was 58%.The expression of bFGF was related to clinical classification and lymph nodes metastasis.The difference was significant among the groups (χ2=3.864 and 3.876,P＜0.05).The expression of bFGF has no significant relationship with some pathological characteristics such as histological types,pathological grade.The positive rate of TGFβ1 was 60%.The expression of TGFl3l had significant relationship with some pathological characteristics such as histological types,clinical classification and lymph nodes metastasis.The difference was significant among the groups (χ2=3.852,4.151 and 4.443,P＜0.05).The expression ofTGFβ1 was not related to pathological grade.The expression of MVD was related to clinical classification and lymph nodes metastasis.The difference was significant among the groups(t=2.312 and 2.865,P＜0.05).The expression of MVD had 110 significant relationship with some pathological characteristics such as histological types and pathological grade.The four of all factors had closed statistical significance between cancerous tissue and para.cancerous tissue.The expressions of VEGF and bFGFwere related to MVD.The difference Was significant among the groups(t=2.518 and 3.679,P＜0.05).The expression of TGFl3,had no significant relationship with MVD.Conclusion VEGF,bFGF and TGFDl have very high expressions in NSCLC.The expression levels of VEGF,bFGF and TGFβ1 are significantly related to the invasive growth and metastasis of NSCLC.VEGF and bFGF play an important role during the tumor neovascularization and tumor metastasis.TGFβ1 may be associated with the decreasing of immunoregulation function and the escape of immune survival.