国际医学寄生虫病杂志
國際醫學寄生蟲病雜誌
국제의학기생충병잡지
INTERNATIONAL JOURNAL OF MEDICAL PARASITIC DISEASES
2011年
3期
154-157
,共4页
仲维霞%屈金辉%王洪法%赵桂华%崔勇
仲維霞%屈金輝%王洪法%趙桂華%崔勇
중유하%굴금휘%왕홍법%조계화%최용
抗菌肽%猪蛔虫%杜氏利什曼原虫%杀伤作用
抗菌肽%豬蛔蟲%杜氏利什曼原蟲%殺傷作用
항균태%저회충%두씨리십만원충%살상작용
Antibacterial peptide%Ascaris suum%Leishmania donovani%Killing effect
目的 研究蛔虫抗菌肽酵母发酵产物对杜氏利什曼原虫的抑杀作用,探讨此抗菌肽可作为治疗黑热病的药物的可能性.方法 培养利什曼原虫至适当浓度,接种于96孔组织培养板中,设实验组和对照组,实验组分别加入10μl浓度为30、60、90、120、150、180μg∥ml的蛔虫抗菌肽酵母发酵产物浓缩上清液,每个浓度重复9孔,对照组加相应体积的对照表达产物,分别在连续培养24、48和72 h后,每孔加入20μl四甲基偶氮唑盐(5 mg∥ml),继续培养4 h,每孔加入100μlformanzan溶解液,孵育4 h左右,在570 am测定吸光度,根据吸光度数值计算杀伤率.结果 实验组按浓度由低到高(30～180μg//ml)对应的杀伤率在培养24 h后为20.12%、41.39%、64.89%、65.14%、66.49%和66.49%,培养48 h后为40.12%、67.34%、75.1l%、82.03%、82.75%和82.75%;培养72 h后为45.89%、65.57%、78.49%、82.58%、85.38%和85.38%.蛔虫抗菌肽发酵产物浓度为150μg/ml时,杀伤作用最大,杀伤率为85.38%,IC50为50μg/ml.结论 蛔虫抗菌肽对利什曼原虫有很强的杀伤作用.
目的 研究蛔蟲抗菌肽酵母髮酵產物對杜氏利什曼原蟲的抑殺作用,探討此抗菌肽可作為治療黑熱病的藥物的可能性.方法 培養利什曼原蟲至適噹濃度,接種于96孔組織培養闆中,設實驗組和對照組,實驗組分彆加入10μl濃度為30、60、90、120、150、180μg∥ml的蛔蟲抗菌肽酵母髮酵產物濃縮上清液,每箇濃度重複9孔,對照組加相應體積的對照錶達產物,分彆在連續培養24、48和72 h後,每孔加入20μl四甲基偶氮唑鹽(5 mg∥ml),繼續培養4 h,每孔加入100μlformanzan溶解液,孵育4 h左右,在570 am測定吸光度,根據吸光度數值計算殺傷率.結果 實驗組按濃度由低到高(30～180μg//ml)對應的殺傷率在培養24 h後為20.12%、41.39%、64.89%、65.14%、66.49%和66.49%,培養48 h後為40.12%、67.34%、75.1l%、82.03%、82.75%和82.75%;培養72 h後為45.89%、65.57%、78.49%、82.58%、85.38%和85.38%.蛔蟲抗菌肽髮酵產物濃度為150μg/ml時,殺傷作用最大,殺傷率為85.38%,IC50為50μg/ml.結論 蛔蟲抗菌肽對利什曼原蟲有很彊的殺傷作用.
목적 연구회충항균태효모발효산물대두씨리십만원충적억살작용,탐토차항균태가작위치료흑열병적약물적가능성.방법 배양리십만원충지괄당농도,접충우96공조직배양판중,설실험조화대조조,실험조분별가입10μl농도위30、60、90、120、150、180μg∥ml적회충항균태효모발효산물농축상청액,매개농도중복9공,대조조가상응체적적대조표체산물,분별재련속배양24、48화72 h후,매공가입20μl사갑기우담서염(5 mg∥ml),계속배양4 h,매공가입100μlformanzan용해액,부육4 h좌우,재570 am측정흡광도,근거흡광도수치계산살상솔.결과 실험조안농도유저도고(30～180μg//ml)대응적살상솔재배양24 h후위20.12%、41.39%、64.89%、65.14%、66.49%화66.49%,배양48 h후위40.12%、67.34%、75.1l%、82.03%、82.75%화82.75%;배양72 h후위45.89%、65.57%、78.49%、82.58%、85.38%화85.38%.회충항균태발효산물농도위150μg/ml시,살상작용최대,살상솔위85.38%,IC50위50μg/ml.결론 회충항균태대리십만원충유흔강적살상작용.
Objective To study the killing effect of yeast fermentation product of Ascaris antibacterial peptide on Leishmania donovani and to investigate the possibility of antibacterial peptide as drug for kala-azar.Methods Leishmania was cultured to appropriate concentration,and was seeded in 96-well tissue culture plate.Experimental test and the negative control hole were designed.Then 10 μl supernatant of induced bacteria expression product was added into experimental hole with concentration of 30,60,90,120,150 and 180μg/ml.All levels were repeated nine holes.Then 20 μl methyl thiazolyl tetrazolium(MTT,5 mg/ml)was added into each hole and culturing for 4 h after culturing for 24,48 and 72 h,and another 100μl of Formanzan was pulsed,absorbance was read at 570 nm after another 4 h cultured.The killing effect is calculate from A570.Results At concentrations of the experimental groups from low to high(30～180μg/ml),the killing effects 12 h after treatment were 20.12%,41.39%,64.89%,65.14%,66.49%,66.49%,48 h after treatment were 40.12%,67.34%,75.11%,82.03%,82.75%,82.75%and 72 h after treatment were 45.89%,65.57%,78.49%,82.58%,85.38%and 85.38%respectively.The most killing effect was 85.38%when the concentration of antibacterial peptide expression product reached 150μg/ml,and the IC50 was 50 μg∥ml.Conclusion Ascaris peptide has a strong killing effect on Leishmania.
