CAJ | 학술논문

目的探讨先天性巨细胞病毒(HCMV)感染的新生儿gB基因分型与临床表现的关系。方法采集67例经PCR方法确诊的有症状的先天性HCMV感染新生儿尿液标本。用巢式PCR方法扩增尿液标本中gB基因片段，用限制性片段长度多态性分析检测gB基因分型。结果 67例病例中最常见的基因型为gB1 (50.7％)，其次为gB3 (23.9％)，gB2 (17.9％)和gB1/gB3混合感染(7.5％)，gB4基因型未检测到。在有肝脏损害的患儿中，gB1基因型最为常见(27/37，73.0％)，高于无肝脏损害的其他有症状感染患儿( 13/30,43.3％；P＜0.05)。结论在有症状的先天性HCMV感染新生儿中最常见的gB基因型为gB1，其次是gB3、gB2及gB4。
목적 탐토선천성거세포병독(HCMV)감염적신생인gB기인분형여림상표현적관계。방법채집67례경PCR방법학진적유증상적선천성HCMV감염신생인뇨액표본。용소식PCR방법확증뇨액표본중gB기인편단，용한제성편단장도다태성분석검측gB기인분형。결과 67례병례중최상견적기인형위gB1 (50.7％)，기차위gB3 (23.9％)，gB2 (17.9％)화gB1/gB3혼합감염(7.5％)，gB4기인형미검측도。재유간장손해적환인중，gB1기인형최위상견(27/37，73.0％)，고우무간장손해적기타유증상감염환인( 13/30,43.3％；P＜0.05)。결론 재유증상적선천성HCMV감염신생인중최상견적gB기인형위gB1，기차시gB3、gB2급gB4。
ObjectiveTo investigate human cytomegalovirus (HCMV) glycoprotein B (gB)genotypes and clinical features in neonates with congenital infections. MethodsUrine samples were obtained from 67 neonates with HCMV infection confirmed by polymerase chain reaction (PCR). The gB gene fragment was amplified by nested PCR. HCMV gB genotyping was detected by restriction fragment length polymorphism. ResultsIn all these cases, the most prevalent genotype was gB1 (50. 7％ ),followed by gB3 (23.9％), gB2 ( 17.9％ ), and gB1/gB3 coinfection ( 7.5％ ) ; gB4 was not found. Moreover, gB1 was more prevalent in infants with liver damage (27/37,73.0％) than in other symptomatic infants without liver damage ( 13/30, 43.3％ ; P ＜ 0. 05 ). ConclusionThe gB1 genotype is the most prevalent in infants with congenital symptomatic HCMV disease, especially in those with liver damage, followed by genotypes gB3, gB2, and gB4.