中华整形外科杂志
中華整形外科雜誌
중화정형외과잡지
CHINESE JOURNAL OF PLASTIC SURGERY
2008年
6期
472-475
,共4页
骨髓间质干细胞%皮肤移植%免疫%白细胞介素2%IFN-γ诱导因子
骨髓間質榦細胞%皮膚移植%免疫%白細胞介素2%IFN-γ誘導因子
골수간질간세포%피부이식%면역%백세포개소2%IFN-γ유도인자
Bone marrow stromal cells%Skin tranaplantation%Immunity%Graft rejection%IL-2%IFN-γ
目的 观察自体骨髓间质干细胞(bone marrow stromal cells,BMSCs)对异基因小鼠皮肤移植的影响.方法 建立C57BL/6至BALB/c小鼠皮肤移植急性排斥模型,密度梯度离心法分离与贴壁法富集BALB/c小鼠BMSCs,自尾静脉输注.实验分3组,每组10只小鼠.A组:正常BALB/c小鼠+自体BMSCs,B组:仅行皮肤移植,C组:皮肤移植后BALB/c小鼠+自体BMSCs.检测各时间段皮肤组织病理表现以及相关细胞因子浓度变化.结果 由密度梯度离心法与贴壁法分离的细胞具有BMSCs的一般特征,C组较B组移植后皮肤免疫排斥反应明显减轻,IL-2、IFN-γ等细胞因子浓度在术后第7天(F=248 954.6,P＜0.05;F=148 311.7,P＜0.05)、第14天(F=117 372.3,P＜0.05:F=126 743.3,P＜0.05)均有降低.结论 异基因小鼠皮肤移植术后输注自体BMSCs可以抑制免疫排斥反应,其机制可能与减少IL-2、IFN-γ等相关细胞因子分泌有关.
目的 觀察自體骨髓間質榦細胞(bone marrow stromal cells,BMSCs)對異基因小鼠皮膚移植的影響.方法 建立C57BL/6至BALB/c小鼠皮膚移植急性排斥模型,密度梯度離心法分離與貼壁法富集BALB/c小鼠BMSCs,自尾靜脈輸註.實驗分3組,每組10隻小鼠.A組:正常BALB/c小鼠+自體BMSCs,B組:僅行皮膚移植,C組:皮膚移植後BALB/c小鼠+自體BMSCs.檢測各時間段皮膚組織病理錶現以及相關細胞因子濃度變化.結果 由密度梯度離心法與貼壁法分離的細胞具有BMSCs的一般特徵,C組較B組移植後皮膚免疫排斥反應明顯減輕,IL-2、IFN-γ等細胞因子濃度在術後第7天(F=248 954.6,P＜0.05;F=148 311.7,P＜0.05)、第14天(F=117 372.3,P＜0.05:F=126 743.3,P＜0.05)均有降低.結論 異基因小鼠皮膚移植術後輸註自體BMSCs可以抑製免疫排斥反應,其機製可能與減少IL-2、IFN-γ等相關細胞因子分泌有關.
목적 관찰자체골수간질간세포(bone marrow stromal cells,BMSCs)대이기인소서피부이식적영향.방법 건립C57BL/6지BALB/c소서피부이식급성배척모형,밀도제도리심법분리여첩벽법부집BALB/c소서BMSCs,자미정맥수주.실험분3조,매조10지소서.A조:정상BALB/c소서+자체BMSCs,B조:부행피부이식,C조:피부이식후BALB/c소서+자체BMSCs.검측각시간단피부조직병리표현이급상관세포인자농도변화.결과 유밀도제도리심법여첩벽법분리적세포구유BMSCs적일반특정,C조교B조이식후피부면역배척반응명현감경,IL-2、IFN-γ등세포인자농도재술후제7천(F=248 954.6,P＜0.05;F=148 311.7,P＜0.05)、제14천(F=117 372.3,P＜0.05:F=126 743.3,P＜0.05)균유강저.결론 이기인소서피부이식술후수주자체BMSCs가이억제면역배척반응,기궤제가능여감소IL-2、IFN-γ등상관세포인자분비유관.
Objective To investigate the effect of recipient derived bone marrow stromal cells(BMSCs)on immunological rejection in mouge allogenetic skin transplantation.Methods The C57BL/6 to BALB/c allogenetic skin transplantation model was created.The bone marrow stromal cells(BMSCs)were isolated from BALB/c by gradient density centrifugation and adhesion separation.The BMSCs were injected back through tail vein.The mouses were divided into three groups as group A(BALB/c+BMSCs),group B(BALB/c with skin transplatation),and group C(BALB/c with skin transplatation+BMSCs).The pathologic examination of the graft was performed and the cytokines such as IL-2,IFN-γ were detected at the different time.Results The attained BMSCs in the experiment had the characteristics of BMSCs.The acute immunological reiection reaction detected by immunohistochemistry staining was alleviated noticeably in group C than that in group B.The concentrations of cytokines IL-2、IFN-γ in group B were lower than that in group C at 7 d(F=248 954.6.P＜0.05;F=148 311.7,P＜0.05)and 14 d(F=117 372.3,P＜0.05;F=126 743.3,P＜0.05)after skin transplantation.Conclusions Recipient derived BMSCs transfusion Call alleviate the acute immunolosical rejection after allogenetic skin transplantation.The possible mechanism maybe related to the inhibitory effect on the secretion of cytokines like IL-2,IFN-γ.
