CAJ | 학술논문

目的观察间充质干细胞(MSC)与不同比例脐血CD34+细胞共移植对NOD/SCID小鼠造血重建的影响,明确MSC与脐血CD34+细胞共移植的最适数量.方法给60Coγ射线照射的雌性NOD/SCID小鼠共移植人MSC和不同比例的脐血CD34+细胞,观察共移植后42 d内小鼠外周血白细胞和血小板变化,并于移植后42 d处死小鼠,用流式细胞术检测外周血、骨髓和脾脏人源细胞含量.结果与单纯脐血CD34+细胞移植相比较:①脐血CD34+细胞与1、5和10倍数量的MSC共移植时,可明显减轻外周血白细胞和皿小板的下降幅度(P<0.01),提前1周使白细胞和血小板恢复至正常水平(P<0.05),三组间差异无统计学意义(P>0.05);②MSC与不同比例的脐血CD34+细胞共移植均可明显提高外周血、骨髓和脾脏造血细胞植入率.比例为10:1时,外周血、骨髓和脾脏中的人源细胞(huCD45+细胞)含量分别增加了(2.8±0.6)倍、(3.5±0.9)倍和(5.2±0.6)倍,增加倍数差异均有统计学意义(P<0.01),达到了最佳的植入效果.结论脐血CD34+细胞与10倍数量的MSC共移植可达到最佳的促进造血重建作用.
목적 관찰간충질간세포(MSC)여불동비례제혈CD34+세포공이식대NOD/SCID소서조혈중건적영향,명학MSC여제혈CD34+세포공이식적최괄수량.방법 급60Coγ사선조사적자성NOD/SCID소서공이식인MSC화불동비례적제혈CD34+세포,관찰공이식후42 d내소서외주혈백세포화혈소판변화,병우이식후42 d처사소서,용류식세포술검측외주혈、골수화비장인원세포함량.결과 여단순제혈CD34+세포이식상비교:①제혈CD34+세포여1、5화10배수량적MSC공이식시,가명현감경외주혈백세포화명소판적하강폭도(P<0.01),제전1주사백세포화혈소판회복지정상수평(P<0.05),삼조간차이무통계학의의(P>0.05);②MSC여불동비례적제혈CD34+세포공이식균가명현제고외주혈、골수화비장조혈세포식입솔.비례위10:1시,외주혈、골수화비장중적인원세포(huCD45+세포)함량분별증가료(2.8±0.6)배、(3.5±0.9)배화(5.2±0.6)배,증가배수차이균유통계학의의(P<0.01),체도료최가적식입효과.결론 제혈CD34+세포여10배수량적MSC공이식가체도최가적촉진조혈중건작용.
Objective To study the effect of co-transplant of human bone marrow mesenchymal stem cells (BMMSCs) and umbilical cord blood (UCB) CD34+ cells on hematopoiesis reconstruction in NOD/SCID mice and to investigate the optimal proportion between the two kind of cells. Methods Female NOD/SCID mice were sublethally irradiated by 60Co γ-ray and transplanted with BMMSCs and different ratios of UCB CD34+ cells. From day +3 till day +42 after transplantation, 20μl peripheral blood (PB) was collected from the retro-orhital plexus of mice weekly, and the variations of WBC and PLT were counted. Mice were sacrificed 42 days after transplantation, and human CD45 positive (huCD45+) cells in PB, BM, and spleen were detected by flow cytometry. Results Compared with transplant of UCB CD34+ cells alone, cothe decrease range (P< 0.01)and led to the recovery of WBC and platelet in PB one week earlier(P<enhanced hematopoietic stem cells (PB, BM and spleen cells) engraftment in recipient mice, and the effect was most pronounced at the ratio of 10: 1. huCD45+ cells in PB, BM and spleen were increased by (2.75±0.63), (3.51 ± 0.86) and (5.18±0.57) fold, respectively (P < 0.01). Conclusion The optimal hematopoiesis reconstruction is achieved by co-transplant of UCB CD34+ cells and BMMSCs at a ratio of 1: 10.