CAJ | 학술논문

目的观察小分子蛋白多糖decorin(DCN)局部注射对兔耳增生性瘢痕的抑制作用.方法新西兰大白兔8只,建立增生性瘢痕动物模型,随机分成两组.两组均于术后第20天、第30天行瘢痕内注射,实验组(A组)注射decorin,对照组(B组)注射生理盐水.末次注射后20 d取材,观察瘢痕增生;苏木素-伊红(HE)染色比较瘢痕增生指数,免疫组织化学检测瘢痕组织中Ⅰ型胶原蛋白表达;逆转录-聚合酶链反应(RT-PCR)检测Ⅰ型前胶原mRNA表达.结果 A组上皮角化良好,瘢痕质地较软,色泽与周围正常皮肤接近.A组瘢痕增生指数为2.8970±0.2066(B组3.5210±0.1960);Ⅰ型胶原蛋白表达值为0.221 20±0.027 06(B组0.33690±0.03074);Ⅰ型前胶原mRNA表达值为0.372 50±0.026 78(B组0.645 60±0.029 94),与B组比较均降低,差异有统计学意义(P＜0.05).结论小分子蛋白多糖decorin局部注射可以抑制早期兔耳腹增生性瘢痕,减轻瘢痕的增生程度.
목적 관찰소분자단백다당decorin(DCN)국부주사대토이증생성반흔적억제작용.방법 신서란대백토8지,건립증생성반흔동물모형,수궤분성량조.량조균우술후제20천、제30천행반흔내주사,실험조(A조)주사decorin,대조조(B조)주사생리염수.말차주사후20 d취재,관찰반흔증생;소목소-이홍(HE)염색비교반흔증생지수,면역조직화학검측반흔조직중Ⅰ형효원단백표체;역전록-취합매련반응(RT-PCR)검측Ⅰ형전효원mRNA표체.결과 A조상피각화량호,반흔질지교연,색택여주위정상피부접근.A조반흔증생지수위2.8970±0.2066(B조3.5210±0.1960);Ⅰ형효원단백표체치위0.221 20±0.027 06(B조0.33690±0.03074);Ⅰ형전효원mRNA표체치위0.372 50±0.026 78(B조0.645 60±0.029 94),여B조비교균강저,차이유통계학의의(P＜0.05).결론 소분자단백다당decorin국부주사가이억제조기토이복증생성반흔,감경반흔적증생정도.
Objective To observe the inhibitory effect of decorin on hypertrophic scar in rabbit ears. Methods Full-thickness excisional wounds, 8 mm in diameter, were made over the ventral side of ears in 8 adult New Zealand white rabbits, and 48 hypertrophic scars in total. Then the rabbits were divided into two groups: group A, experimental group (decorin, 10 mg/L); group B, control group (physiological saline). At the 20th and 30th day after operation, the hypertrophic scars were injected with decorin (group A) and saline (group B) intralesionally. Samples were harvested 20 days after the last injection (50th day) for measurement of scar elevation index (SEI). The expression of type Ⅰ collagen protein and type Ⅰ pro-collagen mRNA was assessed by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) respectively. Results Compared with group B, the scars appeared to be flatter,softer, and lighter in color in group A. The SEI in group A and group B was 2. 8970 ±0. 2066 and 3. 5210 ±0. 1960 respectively. The expression of type Ⅰ collagen protein and type Ⅰ pro-collagen mRNA in group A was 0. 221 20 ±0. 027 06 and 0. 372 50 ±0.026 78 respectively, and that in group B was 0. 336 90 ±0.030 74 and 0.645 60 ±0.029 94 respectively ( all P ＜ 0.05). Conclusion Decorin can inhibit the proliferation process of hypertrophic scar in rabbit ears and remarkably decrease the degree of fibrosis in the scar.