CAJ | 학술논문

目的鉴定内蒙古通辽市分离的虫媒病毒.方法使用血清学及分子生物学方法鉴定内蒙古蚊虫标本分离的病毒,对毒株的部分核苷酸序列进行测定,应用Clustal X软件做碱基配对分析,MEGA软件完成病毒进化分析,DNAStar软件包中的MegAlign完成核苷酸和氨基酸同源性分析.结果在内蒙古通辽市采集凶小库蚊标本分离的病毒经过血清学和分子生物学实验鉴定为版纳病毒(Banna virus,BAV),与版纳病毒部分中国分离株位于同一进化分支中,病毒第12片段核苷酸同源性为89.6%～98.4%,氨基酸同源性为90.4%～98.6%.结论在内蒙古凶小库蚊标本中分离到版纳病毒.
목적 감정내몽고통료시분리적충매병독.방법 사용혈청학급분자생물학방법감정내몽고문충표본분리적병독,대독주적부분핵감산서렬진행측정,응용Clustal X연건주감기배대분석,MEGA연건완성병독진화분석,DNAStar연건포중적MegAlign완성핵감산화안기산동원성분석.결과 재내몽고통료시채집흉소고문표본분리적병독경과혈청학화분자생물학실험감정위판납병독(Banna virus,BAV),여판납병독부분중국분리주위우동일진화분지중,병독제12편단핵감산동원성위89.6%～98.4%,안기산동원성위90.4%～98.6%.결론 재내몽고흉소고문표본중분리도판납병독.
Objective To identify the virus isolated from a mosquito Culex modestus collected from Tongliao city of Inner Mongolia Autonomous Region. Methods A strain of virus isolated from mosquito in Tongliao city was identified by serological and molecular biological methods. The nucleotides of the virus isolate were amplified by RT-PCR, and the products were purified and sequenced. Multiple alignment, phylogenetic and amino acid (AA) analysis were carried out by software Chstal X, MEGA4 and MegAlign (DNAStar) .Results The new isolate was identified to be Banna virus by serological and molecular biological methods. Phylogenetic analysis showed that the Chinese isolates were distributed within one cluster. The homologue of nucleotide and amino acid of 12 segments between the new isolate and other strains isolated from China were 89.6%-98.4% and 90.4%-98.6 %. Conclusion The virus isolated from Culex modestus in Inner Mongolia belonged to Banna virus, and it is the first time that Banna virus was isolated in this region.