中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
11期
1716-1718
,共3页
符伟军%曹磊%徐福强%李钢%王忠新%洪宝发%王晓雄%胡堃%崔福斋%张旭
符偉軍%曹磊%徐福彊%李鋼%王忠新%洪寶髮%王曉雄%鬍堃%崔福齋%張旭
부위군%조뢰%서복강%리강%왕충신%홍보발%왕효웅%호곤%최복재%장욱
输尿管%支架%生物相容性
輸尿管%支架%生物相容性
수뇨관%지가%생물상용성
Ureter%Stent%Biocompatibility
目的 观察携带缓释药物的生物可降解输尿管支架的体内外降解特性,比较输尿管裸支架与输尿管药物支架的生物相容性等差异.方法 将输尿管裸支架与输尿管药物支架分别浸泡于新鲜人体尿液中,观察体外支架降解.将两种输尿管支架分别包埋于18只Wistar大鼠脊柱两侧肌肉内.通过组织切片染色、扫描电镜、相对分子质量测定及质量变化分析体内生物可降解特性差异.结果 输尿管药物支架体外人体尿液浸泡10周时,支架失重率已达35%.切片染色发现组织反应较轻,纤维增生不明显.扫描电镜支架表面失去原有形态,出现大块状脱落物.12周时,降解为细小泥沙样颗粒输尿管裸支架组纤维膜厚(21.24±3.27)μm,输尿管药物支架组纤维膜厚(10.75±3.69)μm,两组比较差异有统计学意义(P＜0.01).结论 与输尿管裸支架比较,输尿管药物支架的降解特性相似,但炎症反应轻,包裹纤维膜厚度更薄,具有良好的组织相容性.
目的 觀察攜帶緩釋藥物的生物可降解輸尿管支架的體內外降解特性,比較輸尿管裸支架與輸尿管藥物支架的生物相容性等差異.方法 將輸尿管裸支架與輸尿管藥物支架分彆浸泡于新鮮人體尿液中,觀察體外支架降解.將兩種輸尿管支架分彆包埋于18隻Wistar大鼠脊柱兩側肌肉內.通過組織切片染色、掃描電鏡、相對分子質量測定及質量變化分析體內生物可降解特性差異.結果 輸尿管藥物支架體外人體尿液浸泡10週時,支架失重率已達35%.切片染色髮現組織反應較輕,纖維增生不明顯.掃描電鏡支架錶麵失去原有形態,齣現大塊狀脫落物.12週時,降解為細小泥沙樣顆粒輸尿管裸支架組纖維膜厚(21.24±3.27)μm,輸尿管藥物支架組纖維膜厚(10.75±3.69)μm,兩組比較差異有統計學意義(P＜0.01).結論 與輸尿管裸支架比較,輸尿管藥物支架的降解特性相似,但炎癥反應輕,包裹纖維膜厚度更薄,具有良好的組織相容性.
목적 관찰휴대완석약물적생물가강해수뇨관지가적체내외강해특성,비교수뇨관라지가여수뇨관약물지가적생물상용성등차이.방법 장수뇨관라지가여수뇨관약물지가분별침포우신선인체뇨액중,관찰체외지가강해.장량충수뇨관지가분별포매우18지Wistar대서척주량측기육내.통과조직절편염색、소묘전경、상대분자질량측정급질량변화분석체내생물가강해특성차이.결과 수뇨관약물지가체외인체뇨액침포10주시,지가실중솔이체35%.절편염색발현조직반응교경,섬유증생불명현.소묘전경지가표면실거원유형태,출현대괴상탈락물.12주시,강해위세소니사양과립수뇨관라지가조섬유막후(21.24±3.27)μm,수뇨관약물지가조섬유막후(10.75±3.69)μm,량조비교차이유통계학의의(P＜0.01).결론 여수뇨관라지가비교,수뇨관약물지가적강해특성상사,단염증반응경,포과섬유막후도경박,구유량호적조직상용성.
Objective To observe the degradation characteristics of the novel drug-eluting biodegradable polylactide ureteral stents in vivo and in vitro, and to compare biocompatibility differences of the pure biodegradable ureteral stents with paclitaxel-eluting biodegradable ureteral stent. Methods The polylactide ureteral stents were dipped into fresh urine specimen and urinary environment simulated in vivo by shocks. And in vivo these two kinds of ureteral stents were implanted in the dorsal muscle of 16 Wistar rats. Histological study was used to investigate the tissue reaction of the material and the differeces of these two kind of ureteral stents were evaluated. Scanning election microscopy (SEM) and measurement of gravity and molecular weight were performed to make out the biodegradation characteristics of stents in vitro and in vivo. Results After being soaked in human urine for 10 weeks, weight of the novel drug-eluting stents was decreased by 35%. Under the SEM, the surface of ureteral stent lost its original shape, shedding a large number of blocks. Histological staining found that fibrosis of drug-eluting biodegradable ureteral stent was mild. After 12 weeks, it was degraded into small sand-like particles. The fiber membrane thickness of pure stent was (21.24 ±3. 27) μm and of drug-eluting stents was ( 10. 75 ±3.69) μm. There was significant difference between two groups (P ＜ 0. 01 ). Conclusion The biodegradation of drug-eluting stents is similar to pure biodegradable ureteral stent, but the inflammatory reaction of the former is minor and the fiber membrane is thinner. The drug-eluting stent has better histocompatibility and can be used for stenting after a ureteral repair.
