CAJ | 학술논문

目的去除B8R对外源抗原免疫原性的影响,为降低载体的免疫优势从而提高疫苗的有效性提供参考.方法利用针对痘苗病毒的pSC11质粒,构建插入OVA的质粒pSC11-OVA,在CV-1细胞中与去除B8R的痘苗病毒重组,筛选纯化获得重组病毒.利用体外培养细胞,检测B8R缺失和外源抗原插入对病毒感染复制特性的影响；利用感染小鼠模型,检测重组病毒诱发的针对OVA的细胞免疫应答和免疫记忆效应；采用体征及神经行为学评分系统,检测重组病毒的毒力.结果 B8R缺失和OVA导入对痘苗病毒的生物学特性无明显影响；去除B8R后,外源性OVA成为显性表位,针对OVA的细胞免疫应答和免疫记忆效应明显增强；去除B8R还可显著降低痘苗病毒的毒力.结论去除B8R可有效降低痘苗病毒的免疫优势效应,增加外源性抗原的免疫原性.去除痘苗病毒本身的显性表位,可为疫苗和基因治疗提供更为有效的载体.
목적 거제B8R대외원항원면역원성적영향,위강저재체적면역우세종이제고역묘적유효성제공삼고.방법 이용침대두묘병독적pSC11질립,구건삽입OVA적질립pSC11-OVA,재CV-1세포중여거제B8R적두묘병독중조,사선순화획득중조병독.이용체외배양세포,검측B8R결실화외원항원삽입대병독감염복제특성적영향；이용감염소서모형,검측중조병독유발적침대OVA적세포면역응답화면역기억효응；채용체정급신경행위학평분계통,검측중조병독적독력.결과 B8R결실화OVA도입대두묘병독적생물학특성무명현영향；거제B8R후,외원성OVA성위현성표위,침대OVA적세포면역응답화면역기억효응명현증강；거제B8R환가현저강저두묘병독적독력.결론 거제B8R가유효강저두묘병독적면역우세효응,증가외원성항원적면역원성.거제두묘병독본신적현성표위,가위역묘화기인치료제공경위유효적재체.
Objective To investigate the antigenicity of foreign antigen in recombinant vaccinia virus (VACV) after elimination of B8R,and provide help to improve the efficacy of VACV-based vaccines,and provide guidance for vaccine design.Methods Transfer vector pSC11-OVA was generated,and OVAgene was in,rted into VACV with B8R deletion.The biological characteristics of recombinant VACV was investigated in vitro,and the immune responses against OVA were tested in vivo.Results The plague phenotypes and growth of wcombinant VACV and its parental strains were essentially identical.Cellular immuneresponse against OVA was augmented in mice infected with B8R-deleted recombinant VACV when comparedwith those infected with B8R-intact recombinant VACV.Conclusion Deletion of B8R and insertion of OVAdoes not affect the biological characteristics of VACV.Immunogenicity of exogenous target antigens can beimproved in VACV with B8R deficiency.Deletion of dominant epitopes may provide a vector with more efficiency for vaccines and gene therapy.